The Detail Information for This Interaction


  • Detail
  • Binding
  • Network
Detail-Interactions:
RAID ID: RRI00000043
RNA/Protein Symbol 1:PTENP1
RNA/Protein Category 1: lncRNA
RNA/Protein Symbol 2: MIR19A(miR-19a)
RNA/Protein Category 2: miRNA
Validated Method: Dual Luciferase reporter assay, RT-PCR, Western blot
Tissue:  
PMID: 20577206 
Detail description:

Within the high homology region, we found perfectly conserved seed matches for the PTEN-targeting miR-17, miR-21, miR-214, miR-19 and miR-26 families (Fig. 1c, Supplementary Fig. 1). To measure the role of these microRNAs on both PTEN and PTENP1 expression, we designed specific PCR primer sets in the non-homologous 3?¡¥UTR regions (Supplementary Fig. 2a,b). In DU145 prostate cancer cells, PTEN-targeting microRNAs miR-19b and miR-20a suppress both PTEN and PTENP1 mRNA abundance (Fig. 1d, Supplementary Fig. 3a). In these cells, a pool of inhibitors of endogenously expressed PTEN-targeting microRNAs (Supplementary Fig. 3b) de-repressed both PTEN and PTENP1 transcript levels (Fig. 1e). The use of chimeric luciferase plasmids indicated the microRNA:PTENP1 interaction was direct (Supplementary Fig. 4a¡§Cc). 

The Predicated Binding Sites between and
By miRanda Structure Match Score Energy Score Location Location
The Predicated Binding Sites between and
By RIsearch Structure Location Location Score
The Predicated Binding Sites of PTENP1
RBPBD 

					

					

						RsiteDB
						
					

						BindN
						
							 

								
					

					

						BindN+
						
							 

								
					

					

					    RNAbindR
						
                              
	 
								
					

					

					    Pprint
						
                            

								
					



				 
	
            			
	

	
    
	
	  
	 

      

	  
Visualization:
	  
	  

	  Select the center nodes:
	   First Node
	   Second Node
	  Both the Nodes	
 
      
Select the Level of Neighbour:
	   First Neighbour
	   Second Neighbour  
	  

	        
	  

	 
	 
	


 
	 

	  The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
	  the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes. 
	  
The 'First Neighbour' represents the sub-network of direct interacting with the center node, 
	  the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node. 
	  Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
	 


	  

	   
	
	

  





	

		

           			
				
	
                   The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
 
				   The Binding page: representing the predicted binding sites and/or constants.
 
				   The Network page: representing the interaction sub-network of interacting RNA/Protein.