The Detail Information for This Interaction


  • Detail
  • Binding
  • Network
Detail-Interactions:
RAID ID: RRI00000037
RNA/Protein Symbol 1:POU5F1B(OCT4-pg1)
RNA/Protein Category 1: lncRNA
RNA/Protein Symbol 2: MIR145(miR-145)
RNA/Protein Category 2: miRNA
Validated Method: Dual Luciferase reporter assay, RT-PCR
Tissue:  
PMID: 20577206 
Detail description:

Alignments of gene and pseudogenes sequences show that microRNA-binding sites are well conserved; for example, the miR-145 binding site on OCT4 and its pseudogenes OCT4-pg1, 3, 4 and 5 (Supplementary Fig. 11a); miR-1 family binding sites on CONNEXIN 43 (CX43) and its pseudogene (Supplementary Fig. 11b). 

The Predicated Binding Sites between MIR145 and POU5F1B
By miRanda Structure Match Score Energy Score MIR145 Location POU5F1B Location 
   Query:    3' ucCCUAAGGACCCUUUUGACCug 5'
                  ||| | :||||: ||||||  
   Ref:      5' ggGGAGU-UUGGGGCAACUGGuu 3'
147.00 -28.11 3-22 138-159 
   Query:    3' ucccUAAG-GA-CCCUU--UUGACCUg 5'
                    |||| ||  ||||  ||:|||: 
   Ref:      5' cuucAUUCACUAAGGAAGGAAUUGGGa 3'
124.00 -14.66 2-20 93-119 
   Query:    3' ucccuaaggacCCUUUUGACCUg 5'
                           | :|| ||||| 
   Ref:      5' agcuagggaaaGAGAACCUGGAg 3'
116.00 -17.24 2-13 42-64
The Predicated Binding Sites between MIR145 and POU5F1B
By RIsearch Structure MIR145 Location POU5F1B Location Score 
Query:    3' CCAGUUUUCCCAGGAAUCCCU 5'
          |||||| :||||::  ||||:
Target:   5' GGUCAACGGGGUUUG-AGGGG 3'
3-23 138-157 -22.80
The Predicated Binding Sites of POU5F1B
RBPBD 

					

					

						RsiteDB
						
					

						BindN
						
							 

								
					

					

						BindN+
						
							 

								
					

					

					    RNAbindR
						
                              
	 
								
					

					

					    Pprint
						
                            

								
					



				 
	
            			
	

	
    
	
	  
	 

      

	  
Visualization:
	  
	  

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	  The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
	  the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes. 
	  
The 'First Neighbour' represents the sub-network of direct interacting with the center node, 
	  the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node. 
	  Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
	 


	  

	   
	
	

  





	

		

           			
				
	
                   The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
 
				   The Binding page: representing the predicted binding sites and/or constants.
 
				   The Network page: representing the interaction sub-network of interacting RNA/Protein.