Detail-Interactions: | |
RAID ID: | RRI00000009 |
---|---|
RNA/Protein Symbol 1: | non-coding CD44 3`-UTR |
RNA/Protein Category 1: | lncRNA |
RNA/Protein Symbol 2: | MIR216A(miR-216a) |
RNA/Protein Category 2: | miRNA |
Validated Method: | Luciferase reporter assay, Western blot, Immunohistochemistry |
Tissue: | |
PMID: | 21149267 |
Detail description: | Here, we show the 3`-UTR of CD44 is able to antagonize cytoplasmic miRNAs, and result in the increased translation of CD44 and downstream target mRNA, CDC42. Computational algorithms have predicted three miRNAs, miR-216a, miR-330 and miR-608, can bind to both the CD44 and CDC42 3`-UTRs. This was confirmed with luciferase assays, western blotting and immunohistochemical staining and correlated with a series of siRNA assays. Thus, the non-coding CD44 3`-UTR serves as a competitor for miRNA binding and subsequently inactivates miRNA functions, by freeing the target mRNAs from being repressed. |
The Predicated Binding Sites between and | |||||
By miRanda | Structure | Match Score | Energy Score | Location | Location |
The Predicated Binding Sites between and | ||||
By RIsearch | Structure | Location | Location | Score |
The Predicated Binding Sites of non-coding 3`-UTR | |
RBPBD | |
RsiteDB | |
BindN | |
BindN+ | |
RNAbindR | |
Pprint |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.