| Detail-Interactions: | |
| RAID ID: | RPI00001619 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | EPDR1(UCC1) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-106 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and EPDR1 | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | EPDR1 Location |
| The Predicated Binding Sites between TERC and EPDR1 | ||||
| By RIsearch | Structure | TERC Location | EPDR1 Location | Score |
| The Predicated Binding Sites of EPDR1 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|Q9UM22|EPDR1_HUMAN Mammalian ependymin-related protein 1 OS=Homo sapiens GN=EPDR1 PE=1 SV=2 Sequence: MPGRAPLRTVPGALGAWLLGGLWAWTLCGLCSLGAVGAPRPCQAPQQWEGRQVMYQQSSG Prediction: ---+-+-+-------------------------------+--+-------+-----++++ Confidence: 544935372534577859888949659779827554342832642223366273226797 Sequence: RNSRALLSYDGLNQRVRVLDERKALIPCKRLFEYILLYKDGVMFQIDQATKQCSKMTLTQ Prediction: ++++------------+------------+-------------------++++++----- Confidence: 977934532443232854568327344825988599966888686972566556742624 Sequence: PWDPLDIPQNSTFEDQYSIGGPQEQITVQEWSDRKSARSYETWIGIYTVKDCYPVQETFT Prediction: -----------+-------------------+-+++++++-------------------- Confidence: 768797852334444443533233273834253898596542298844727837945474 Sequence: INYSVILSTRFFDIQLGIKDPSVFTPPSTCQMAQLEKMSEDCSW Prediction: ---------+--------------+--++-+-----+------- Confidence: 94336763258769487727427443254443528445457744 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q9UM22|EPDR1_HUMAN Mammalian ependymin-related protein 1 OS=Homo sapiens GN=EPDR1 PE=1 SV=2 Sequence: MPGRAPLRTVPGALGAWLLGGLWAWTLCGLCSLGAVGAPRPCQAPQQWEGRQVMYQQSSG Prediction: -+++++-++-+----------------------------+++++---+----------+- Confidence: 135834364343366969788859589979867642142755631124333673462132 Sequence: RNSRALLSYDGLNQRVRVLDERKALIPCKRLFEYILLYKDGVMFQIDQATKQCSKMTLTQ Prediction: +--+----------+-+------------+-------------------+++++----+- Confidence: 423436531312515744656334532313789979986868778861644635131244 Sequence: PWDPLDIPQNSTFEDQYSIGGPQEQITVQEWSDRKSARSYETWIGIYTVKDCYPVQETFT Prediction: ------------------------+---+-++-++------------------------- Confidence: 244563722542589664343462372535434442512288688965516555826363 Sequence: INYSVILSTRFFDIQLGIKDPSVFTPPSTCQMAQLEKMSEDCSW Prediction: ---------------------------+--------------+- Confidence: 95759998736779789936826458342543888747745332 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q9UM22|EPDR1 Sequence: MPGRAPLRTVPGALGAWLLGGLWAWTLCGLCSLGAVGAPRPCQAPQQWEGRQVMYQQSSG Prediction: ++++++-++-++-------+-------------------++++-++-+------------ Confidence: 567966485155411242250020000000000011223676647635314001001102 Sequence: RNSRALLSYDGLNQRVRVLDERKALIPCKRLFEYILLYKDGVMFQIDQATKQCSKMTLTQ Prediction: ---+++-+++++++---------------------------------------+------ Confidence: 212565455655554140000000001101000000111110000013443325122123 Sequence: PWDPLDIPQNSTFEDQYSIGGPQEQITVQEWSDRKSARSYETWIGIYTVKDCYPVQETFT Prediction: ------------------------------------------------------------ Confidence: 403000010111000000000010000000000000000000000000000001020000 Sequence: INYSVILSTRFFDIQLGIKDPSVFTPPSTCQMAQLEKMSEDCSW Prediction: --------------------------+------------------ Confidence: 001100000010000000121312125101100100000000210 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MPGRAPLRTVPGALGAWLLGGLWAWTLCGLCSLGAVGAPRPCQAPQQWEGRQVMYQQSSG Prediction: -+++--+--------------------------------++++-+-----------+++- Confidence: 347723330020000000000000000000001213203654624212221211034543 Sequence: RNSRALLSYDGLNQRVRVLDERKALIPCKRLFEYILLYKDGVMFQIDQATKQCSKMTLTQ Prediction: +-----+----------------------+-------------------+++-++----- Confidence: 320202420121133020121323013003000000000000000012355424323021 Sequence: PWDPLDIPQNSTFEDQYSIGGPQEQITVQEWSDRKSARSYETWIGIYTVKDCYPVQETFT Prediction: +-------------------+-----+-+-+++++----------+-------------- Confidence: 310000000000000002205200104052654742211122130331200000000000 Sequence: INYSVILSTRFFDIQLGIKDPSVFTPPSTCQMAQLEKMSEDCSW Prediction: ----------------------------------------+--- Confidence: 00000000232000000000010000000000000000004211 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.