| Detail-Interactions: | |
| RAID ID: | RPI00001618 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | TNFRSF1A |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-105 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and TNFRSF1A | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | TNFRSF1A Location |
| The Predicated Binding Sites between TERC and TNFRSF1A | ||||
| By RIsearch | Structure | TERC Location | TNFRSF1A Location | Score |
| The Predicated Binding Sites of TNFRSF1A | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P19438|TNR1A_HUMAN Tumor necrosis factor receptor superfamily member 1A OS=Homo sapiens GN=TNFRSF1A PE=1 SV=1 Sequence: MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCT Prediction: ------------------------------------++++-+-----++-+----+---+ Confidence: 777328669998999999998936269768963533757735322246645333355335 Sequence: KCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVD Prediction: +-++-++-------+-+-+--++-++-+-+-+----+---+-++-++---+----+-+-- Confidence: 735825572467335352536753553625453337645642683772456946252464 Sequence: RDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECV Prediction: +------+++++++----------------------+---+--+---------------- Confidence: 572432399987952345799799694868452835532352345528585569297558 Sequence: SCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWK Prediction: --++-+++----+---------+-+--+-++-----------------------++++++ Confidence: 235636764343587764963855523635588889999999999599997873765868 Sequence: SKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYT Prediction: ++-++----++++-+------+++++-----+-+-+---+-+---++--+++-+++++++ Confidence: 776445654878526253523566853322363525233445674545266828886665 Sequence: PGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLY Prediction: +---------++----------------------------------+-++---------- Confidence: 533542334356343353256659782777245732463255424253654324336283 Sequence: AVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLEL Prediction: ---------+-----++---------+----+-+--+--------++++++++++-+--- Confidence: 799968438562357558553454855835243646736222453547998698545658 Sequence: LGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR Prediction: --+--+-------------------------+--+ Confidence: 86599599898999999999877476533735577 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P19438|TNR1A_HUMAN Tumor necrosis factor receptor superfamily member 1A OS=Homo sapiens GN=TNFRSF1A PE=1 SV=1 Sequence: MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCT Prediction: ------------------------------------------------------------ Confidence: 348324568888888999986936357566751333232484676747137533324333 Sequence: KCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVD Prediction: -++++-+----------------------------------------------------- Confidence: 255441552254422434156343543532833378155242121222467988553222 Sequence: RDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECV Prediction: -------+-+-------------------------------------------------- Confidence: 343443462422242335557657766748434844445423254423142545563657 Sequence: SCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWK Prediction: ---+-+-+------------------++-+------------------------+-++-- Confidence: 453313443688477996961714225444455577888988898989796553514512 Sequence: SKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYT Prediction: ---------+-------------------------------------------------- Confidence: 226446463315356887962744448835737888888785789868576473553752 Sequence: PGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLY Prediction: ------------------+----------------------------------------- Confidence: 475553677464766645324678778888885853545445453445446889888898 Sequence: AVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLEL Prediction: ------+--+++---++-----------+----+--++---------++++-++--+--- Confidence: 899654512554136446321214861834213754635416455216665193253766 Sequence: LGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR Prediction: -------------------------------+--+ Confidence: 78689398897789679996643222331425448 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P19438|TNFRSF1A Sequence: MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCT Prediction: -+++------------------------------------------------------++ Confidence: 465642222010000000000000000000011111202101101100000001332357 Sequence: KCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVD Prediction: +++++++---------------------------------+++--++-------+-++++ Confidence: 799669621422223223333110112111010100031265743651411111645766 Sequence: RDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECV Prediction: ++++++-++++++------------++-+---+-------+-----++--+--------- Confidence: 875556456875732101001111355373425133433254333255315322101012 Sequence: SCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWK Prediction: -------------------------+---------------------------+++++++ Confidence: 021443433110200012201344454434342012021002111211123236579989 Sequence: SKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYT Prediction: +++++++++++------------------------------------------------- Confidence: 898577567754000000000000000000000000000000000000000000000000 Sequence: PGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLY Prediction: ------------------------------------------------------------ Confidence: 000000000000000011100000000000001001011000000000000000001100 Sequence: AVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLEL Prediction: ---------+-+++-++---+------------+--+++-+++--++-+-+-+--+-+-+ Confidence: 000000112735751663425442110010123642567257533764646483474535 Sequence: LGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR Prediction: -------------------------------+--+- Confidence: 101001111223001200000114433321451170 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCT Prediction: --------------------------+--------++++++-+-----------++++++ Confidence: 012220000000000000000012123212013334445343403112110011544445 Sequence: KCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVD Prediction: +++++++++-+++++++------------+-----+++++-+++++-++----+++++++ Confidence: 899999875353467760000000000223000125533417456934400003648898 Sequence: RDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECV Prediction: ++++-++++++++------------+-----------++++++++-+-++-+------++ Confidence: 993738899684400203010000043222011010044465545052443301322153 Sequence: SCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWK Prediction: ----++++++++-----------------+------------------------+--+-- Confidence: 011356996643220000000000122034100000000000000000001110411301 Sequence: SKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYT Prediction: +++----+++-------------------------------------------------- Confidence: 653222346323122000000000002210000000000000001010000000000000 Sequence: PGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLY Prediction: ------------------+---+------------------------------------- Confidence: 000000200002000002423140000000000000000000000010100000000001 Sequence: AVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLEL Prediction: ---------++----++-++--++------+++++++--+----+-+++++++++++--- Confidence: 000100122642000343653253012021445634400320025337798795553300 Sequence: LGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR Prediction: ----------------------------------- Confidence: 20100111000000020000002313201011103 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.