Detail

Detail-Interactions:
RAID ID:	RPI00001617
RNA/Protein Symbol 1:	TERC(htr)
RNA/Protein Category 1:	snoRNA
RNA/Protein Symbol 2:	THBS1
RNA/Protein Category 2:	Protein
Validated Method:	quantitative fluorescent reverse transcription polymerase chain reaction
Tissue:	bladder cancer cell line biu-104
PMID:	20556588
Detail description:	The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1).

Detail-Interactions:

RAID ID:

RPI00001617

RNA/Protein Symbol 1:

TERC(htr)

RNA/Protein Category 1:

snoRNA

RNA/Protein Symbol 2:

THBS1

RNA/Protein Category 2:

Protein

Validated Method:

quantitative fluorescent reverse transcription polymerase chain reaction

Tissue:

bladder cancer cell line biu-104

PMID:

20556588

Detail description:

The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1).

>sp|P07996|TSP1_HUMAN Thrombospondin-1 OS=Homo sapiens GN=THBS1 PE=1 SV=2 Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: -------------------+-++++---------------++++-++--+++--+++--+ Confidence: 898959899999989642375455533239999999333299892983475522676456 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------+--+--------+++++++-+-----++-++------- Confidence: 867968855846739699978777367778682675487593534365552462284277 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: -+-+--+-----+---+------------+-+-++--------+---------------- Confidence: 242833753637552453368398888876452558275947766372867627449586 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: --------+---+--+-+--+-----------------------+--+-++++++++--- Confidence: 879326545585646676548335254847785692675722534476475587766677 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: -------+-++++-+-++-+-+-+--------------------+--+----+---+-++ Confidence: 298455655577529245262526452877782663832778685378266246324665 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: ------------++---------+++----------------------+----------- Confidence: 423542674237863255363346572325337646246353336264527254775632 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: ----------+----------+---------+++--++-+++-++-++-+-+-++++-++ Confidence: 726456355457233346345435235322356643852667286257242836588288 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: ++---+---++-+++--+-------+-----------+-+--++++++-+-+--+-+-+- Confidence: 854264353564555345433253254272674457258632756668262723625572 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: ++--++--------+--------------++++++++-++--+-+--------------- Confidence: 683386454372445522376777382535666886826822527334276665926536 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: --------------------+-++++--+-+----++-++-+------------------ Confidence: 523564385667236756476366652243544536527625272745976848967866 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: ------+--+++--+-------+-+-++---------+-+++--+-++-++--++--+++ Confidence: 442332642444344366453474625445526743455477227386255245533684 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: -+--+---------+---+--+--+------------------------+++-++-+--+ Confidence: 384265543232526634723644478758683574546675997883656637745334 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: --++-++-+---------------------+----------++------------+-++- Confidence: 626526424527776586676655625255454436432346522527655627253452 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: ----+-++-+-------------------------------+------------------ Confidence: 223253443524354745648677755462434439336335323656667474363744 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: ------++--+-----------------------------++----+--+---------- Confidence: 454242552466532353437855652332653762732745322363374244356776 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: ------+---+---+--++-+++--+-+--+--------------------------++- Confidence: 775364542466634225626642293533666665968899889889695842226647 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: -------++++++------+---+------------------------------+--+-- Confidence: 357485375778533237765365476238465477676387868968372654424554 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: ----------++++----------++---++-+++++++----+--++++-+++--+--- Confidence: 438869672277566269985382442326726967475242664554672655376467 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: -----++++-++-----+-------+--+-+-+++-++---+------++--------+- Confidence: 423326856684423565376356645253836662784477888346454254332453 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: --+-+-+-----------------+--+-- Confidence: 225243776579878999996682436646 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest).

>sp|P07996|TSP1_HUMAN Thrombospondin-1 OS=Homo sapiens GN=THBS1 PE=1 SV=2 Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: -------------------+--------------------+++-+++--++-+++++--- Confidence: 237637855569959774434555468789999979848554414582475156545323 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------+---------+-+--+++++------++---------- Confidence: 755965932714538768738836439959583652356673255385333222379798 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: --+++-------+---+--------------+-++--------+---------------- Confidence: 313543463675632251244385798761341448172813746692845223448489 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: -----------------+--++----+--------+------------------------ Confidence: 959589997899768376337322233737995793876753755984625657988999 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: ------------------------------------------------------------ Confidence: 899869747434875675687743582767762532423765671873699988575725 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: ------------------------------------------------------------ Confidence: 887997998987275144523534455676588864255221226377455668779867 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: ------------------------------------+----------------------- Confidence: 997997677546547688689888779678653354345244613743675367744664 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: ------------------------------------------------------------ Confidence: 346797868767567885667897898886863536358485544643657477778768 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: ------------------------------------------------------------ Confidence: 737825784685754786896998886654432433265446733689689999888879 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: ------------------------------------------------------------ Confidence: 898587996877778688785754374675687866743575886766779758988977 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: ------------------------------------------------------------ Confidence: 633354244435343555523425577699998988978766745856956774353355 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: -----------------+------------------------------------------ Confidence: 537444633344432424242334568759687798978988887523622454576355 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: ------------------------------------------------------------ Confidence: 551353443755643586798877867788458646331554779878986547526554 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: -------------------------------------+---------------------- Confidence: 547979875346458947989488678595474116247524846665644562366643 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: -------------------------+++----------+-------+------------- Confidence: 275236772556456243647765435412432542834531552542122494677742 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: ---+--+++-+---+--+-------++------------------------------+-- Confidence: 224423465282315224232121144263368684656768825434274946112723 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: -------++------++----+----------------------------+--------- Confidence: 338364334211222333422321246125122226344546224836334625127175 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: -----------+++-+-----------------+---------+--+++++++++-++-- Confidence: 413577354115757423512373121243352711512262574455775766738544 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: ++--------+--++--+++++++++--+-+-+++-++++-+---+-------------- Confidence: 334222123474246139845653461151525551654657535522245444346425 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: -++++++-----------------+--+-- Confidence: 244554831235435628962262315612 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest).

>P07996|THBS1 Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: -+---+----------------------------------++++--++-+---++---+- Confidence: 282147110101021211100000000000220100041255562475174145543354 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------------------+--++-+-+-------+++------- Confidence: 011000000000000000000020001000012163367454610112245563411002 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: -+-+-++---+-+--++-+-----------++++-------------------------- Confidence: 153637723361744574501201102114566540102011020413113220000100 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: -----------------+--+++--++--------+------------------------ Confidence: 020000000000011205216583466113101405131102001101220000000000 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: -----------------------------------------------+---++------- Confidence: 000000000000100100000000000001010100021003104206320572233124 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: -----+------------------------------------++++++++---+------ Confidence: 011235111110011212222001211121322242444444665765672448334324 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: ---------------------------------+--+--+-++++-+++-+++------- Confidence: 110000101021010000002020210112122522544747686355646863431110 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: -----+---------------+-------+++++++++-++---+-+------------- Confidence: 103016120111101142113523134438767676774864336454143313121032 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: -+-----------+-+-+-------++++++-+-+-++-++++++++++------++-+- Confidence: 151330212113372535324244457757645463852677766675643421257354 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: +------------+-++-+--------+-++---++-++--+--+--------------- Confidence: 521211102122454654612222323645642465456448445411120110102113 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: --++-++--+----------++----+-----------+--+++-+++-+---------- Confidence: 147546643544243321235533235122210122136427653568454442324323 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: -------+------+--+++-++-+++-+----------------------+-++----+ Confidence: 211244253231447225773754787354234323332121341113224745533445 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: -+++++----------------------------+-----------------------+- Confidence: 468558432122112112032113210101102261143132111002012112414150 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: -+--------------------------------+--+-+++----+------+-+-+-+ Confidence: 350312010123111001121113100101112251474766342161123236463625 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: +-+---------------------+----------+--+--------------------- Confidence: 715411312311111100001432521143332305116132112114112100000131 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: --------------+--+-----+-+-------+-------------------------- Confidence: 220111311332036246224225354404414532210010000100112041131100 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: ------------------------------------------------------------ Confidence: 000002121222010011013103102202424230001000000000101000000100 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: -------+-+--++------------------++----++---+--+-++++++---+-- Confidence: 210113254534561022224312211331425622225712153364878567302620 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: ------++--+--++-++++-++++++++++--++-++-----------------+---- Confidence: 331141554273177258664877585565843774564303040430110004263211 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: +--++-+-+---+---+----+-+-+-+++- Confidence: 5237549362436242522347274835650 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest).

Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: +---------------------------------------+++++++++++++++----- Confidence: 431000000000000122322221000100000000000068564556473697530000 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------------------+--+++++-+----+--+-------+ Confidence: 001011003110020120000000100000202041054585251103321523320024 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: -++++++-++--+-+++++++--------+-+-++-+-+----+-------++------- Confidence: 379769528411523764353000002005260430415120030002000480001000 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: -------------+++-+++++++-+-+-+------------------------------ Confidence: 011000000031153539339998363524000000000011200001221222210000 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: ----------------------------------------------------------++ Confidence: 001111033020011001112321122011000003031000103122100000102255 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: +++++++---+---+---------------+------+++++++++++-+--+++-+--- Confidence: 549547411240125203001303220000300002157949889443151254523222 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: -----+-+++++++-----------------+++++++++++++++-+++++-----+-- Confidence: 000014144565340200000000000102265567587765863335674632020401 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: ------+++--------------++-+++++++++++++++-+++--++-++--+++-++ Confidence: 201122434320000000000024324456685767755561486336429522574366 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: ++--+++++++------+-++-+++++++++++++-++---++-++++-++-++++-++- Confidence: 673358766632020003046058487695987783651334426655244146531440 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: --++++++++++++++++--++++--+++++---++++++++++++++--++-+++++++ Confidence: 024458576464784754213766327665312157699977766864235516575497 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: ++++-++-++++--+-+--+++++++++-+----------+-----+--+-+++++++++ Confidence: 665436434755335232245577634513321102230052222242343564355558 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: +-+++++++++++++-+++++++-+++------+-++++-++-+--++---+--++---+ Confidence: 513433575786787347748842476310112334443346351254212431452133 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: -+-+---------------------+----+++-+++-+-+++----+-------+++++ Confidence: 351423200100101000011000040021457365426165423225300101365654 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: ++---+--+--+++---------++-+++--+-++--+--++--+--+--+--------- Confidence: 451026214235343010001103705543252561351344334025224100113001 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: --------------+-+-++--+-+++-++--+--------------------------- Confidence: 000020000000213340341142444244204312002003103212001000000000 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: ----+-+++-+---+-+++--++++--+-------------------------------- Confidence: 010041346291115246421545432300001000000000010000000000020322 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: ----+++----------------------------------------------------- Confidence: 000145430231020320000001000101113100100000000000301000100100 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: --------++++++-----------------------+--------+++++++++--+-- Confidence: 010001204544650010000000002000000023033321120056669878503400 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: +--++-----+--++--+--+++-+-+++-+-++++-----+-+-+-------------- Confidence: 330382220082334219216652415341503686300204030300000000000010 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: ---++++-++---------------+--+- Confidence: 112335515420000000000001240233 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest).

The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.

The Predicated Binding Sites between TERC and THBS1
By miRanda	Structure	Match Score	Energy Score	TERC Location	THBS1 Location

The Predicated Binding Sites between TERC and THBS1
By RIsearch	Structure	TERC Location	THBS1 Location	Score

The Predicated Binding Sites of THBS1
RBPBD
RsiteDB
BindN	>sp\|P07996\|TSP1_HUMAN Thrombospondin-1 OS=Homo sapiens GN=THBS1 PE=1 SV=2 Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: -------------------+-++++---------------++++-++--+++--+++--+ Confidence: 898959899999989642375455533239999999333299892983475522676456 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------+--+--------+++++++-+-----++-++------- Confidence: 867968855846739699978777367778682675487593534365552462284277 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: -+-+--+-----+---+------------+-+-++--------+---------------- Confidence: 242833753637552453368398888876452558275947766372867627449586 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: --------+---+--+-+--+-----------------------+--+-++++++++--- Confidence: 879326545585646676548335254847785692675722534476475587766677 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: -------+-++++-+-++-+-+-+--------------------+--+----+---+-++ Confidence: 298455655577529245262526452877782663832778685378266246324665 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: ------------++---------+++----------------------+----------- Confidence: 423542674237863255363346572325337646246353336264527254775632 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: ----------+----------+---------+++--++-+++-++-++-+-+-++++-++ Confidence: 726456355457233346345435235322356643852667286257242836588288 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: ++---+---++-+++--+-------+-----------+-+--++++++-+-+--+-+-+- Confidence: 854264353564555345433253254272674457258632756668262723625572 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: ++--++--------+--------------++++++++-++--+-+--------------- Confidence: 683386454372445522376777382535666886826822527334276665926536 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: --------------------+-++++--+-+----++-++-+------------------ Confidence: 523564385667236756476366652243544536527625272745976848967866 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: ------+--+++--+-------+-+-++---------+-+++--+-++-++--++--+++ Confidence: 442332642444344366453474625445526743455477227386255245533684 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: -+--+---------+---+--+--+------------------------+++-++-+--+ Confidence: 384265543232526634723644478758683574546675997883656637745334 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: --++-++-+---------------------+----------++------------+-++- Confidence: 626526424527776586676655625255454436432346522527655627253452 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: ----+-++-+-------------------------------+------------------ Confidence: 223253443524354745648677755462434439336335323656667474363744 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: ------++--+-----------------------------++----+--+---------- Confidence: 454242552466532353437855652332653762732745322363374244356776 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: ------+---+---+--++-+++--+-+--+--------------------------++- Confidence: 775364542466634225626642293533666665968899889889695842226647 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: -------++++++------+---+------------------------------+--+-- Confidence: 357485375778533237765365476238465477676387868968372654424554 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: ----------++++----------++---++-+++++++----+--++++-+++--+--- Confidence: 438869672277566269985382442326726967475242664554672655376467 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: -----++++-++-----+-------+--+-+-+++-++---+------++--------+- Confidence: 423326856684423565376356645253836662784477888346454254332453 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: --+-+-+-----------------+--+-- Confidence: 225243776579878999996682436646 * Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. * Confidence: from level 0 (lowest) to level 9 (highest).
BindN+	>sp\|P07996\|TSP1_HUMAN Thrombospondin-1 OS=Homo sapiens GN=THBS1 PE=1 SV=2 Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: -------------------+--------------------+++-+++--++-+++++--- Confidence: 237637855569959774434555468789999979848554414582475156545323 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------+---------+-+--+++++------++---------- Confidence: 755965932714538768738836439959583652356673255385333222379798 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: --+++-------+---+--------------+-++--------+---------------- Confidence: 313543463675632251244385798761341448172813746692845223448489 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: -----------------+--++----+--------+------------------------ Confidence: 959589997899768376337322233737995793876753755984625657988999 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: ------------------------------------------------------------ Confidence: 899869747434875675687743582767762532423765671873699988575725 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: ------------------------------------------------------------ Confidence: 887997998987275144523534455676588864255221226377455668779867 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: ------------------------------------+----------------------- Confidence: 997997677546547688689888779678653354345244613743675367744664 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: ------------------------------------------------------------ Confidence: 346797868767567885667897898886863536358485544643657477778768 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: ------------------------------------------------------------ Confidence: 737825784685754786896998886654432433265446733689689999888879 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: ------------------------------------------------------------ Confidence: 898587996877778688785754374675687866743575886766779758988977 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: ------------------------------------------------------------ Confidence: 633354244435343555523425577699998988978766745856956774353355 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: -----------------+------------------------------------------ Confidence: 537444633344432424242334568759687798978988887523622454576355 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: ------------------------------------------------------------ Confidence: 551353443755643586798877867788458646331554779878986547526554 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: -------------------------------------+---------------------- Confidence: 547979875346458947989488678595474116247524846665644562366643 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: -------------------------+++----------+-------+------------- Confidence: 275236772556456243647765435412432542834531552542122494677742 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: ---+--+++-+---+--+-------++------------------------------+-- Confidence: 224423465282315224232121144263368684656768825434274946112723 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: -------++------++----+----------------------------+--------- Confidence: 338364334211222333422321246125122226344546224836334625127175 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: -----------+++-+-----------------+---------+--+++++++++-++-- Confidence: 413577354115757423512373121243352711512262574455775766738544 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: ++--------+--++--+++++++++--+-+-+++-++++-+---+-------------- Confidence: 334222123474246139845653461151525551654657535522245444346425 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: -++++++-----------------+--+-- Confidence: 244554831235435628962262315612 * Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. * Confidence: from level 0 (lowest) to level 9 (highest).
RNAbindR	>P07996\|THBS1 Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: -+---+----------------------------------++++--++-+---++---+- Confidence: 282147110101021211100000000000220100041255562475174145543354 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------------------+--++-+-+-------+++------- Confidence: 011000000000000000000020001000012163367454610112245563411002 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: -+-+-++---+-+--++-+-----------++++-------------------------- Confidence: 153637723361744574501201102114566540102011020413113220000100 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: -----------------+--+++--++--------+------------------------ Confidence: 020000000000011205216583466113101405131102001101220000000000 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: -----------------------------------------------+---++------- Confidence: 000000000000100100000000000001010100021003104206320572233124 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: -----+------------------------------------++++++++---+------ Confidence: 011235111110011212222001211121322242444444665765672448334324 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: ---------------------------------+--+--+-++++-+++-+++------- Confidence: 110000101021010000002020210112122522544747686355646863431110 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: -----+---------------+-------+++++++++-++---+-+------------- Confidence: 103016120111101142113523134438767676774864336454143313121032 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: -+-----------+-+-+-------++++++-+-+-++-++++++++++------++-+- Confidence: 151330212113372535324244457757645463852677766675643421257354 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: +------------+-++-+--------+-++---++-++--+--+--------------- Confidence: 521211102122454654612222323645642465456448445411120110102113 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: --++-++--+----------++----+-----------+--+++-+++-+---------- Confidence: 147546643544243321235533235122210122136427653568454442324323 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: -------+------+--+++-++-+++-+----------------------+-++----+ Confidence: 211244253231447225773754787354234323332121341113224745533445 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: -+++++----------------------------+-----------------------+- Confidence: 468558432122112112032113210101102261143132111002012112414150 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: -+--------------------------------+--+-+++----+------+-+-+-+ Confidence: 350312010123111001121113100101112251474766342161123236463625 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: +-+---------------------+----------+--+--------------------- Confidence: 715411312311111100001432521143332305116132112114112100000131 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: --------------+--+-----+-+-------+-------------------------- Confidence: 220111311332036246224225354404414532210010000100112041131100 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: ------------------------------------------------------------ Confidence: 000002121222010011013103102202424230001000000000101000000100 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: -------+-+--++------------------++----++---+--+-++++++---+-- Confidence: 210113254534561022224312211331425622225712153364878567302620 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: ------++--+--++-++++-++++++++++--++-++-----------------+---- Confidence: 331141554273177258664877585565843774564303040430110004263211 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: +--++-+-+---+---+----+-+-+-+++- Confidence: 5237549362436242522347274835650 * Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. * Confidence: from level 0 (lowest) to level 9 (highest).
Pprint	Sequence: MGLAWGLGVLFLMHVCGTNRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFR Prediction: +---------------------------------------+++++++++++++++----- Confidence: 431000000000000122322221000100000000000068564556473697530000 Sequence: IEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVV Prediction: ----------------------------------+--+++++-+----+--+-------+ Confidence: 001011003110020120000000100000202041054585251103321523320024 Sequence: SNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELD Prediction: -++++++-++--+-+++++++--------+-+-++-+-+----+-------++------- Confidence: 379769528411523764353000002005260430415120030002000480001000 Sequence: VPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLL Prediction: -------------+++-+++++++-+-+-+------------------------------ Confidence: 011000000031153539339998363524000000000011200001221222210000 Sequence: TLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRK Prediction: ----------------------------------------------------------++ Confidence: 001111033020011001112321122011000003031000103122100000102255 Sequence: VTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSN Prediction: +++++++---+---+---------------+------+++++++++++-+--+++-+--- Confidence: 549547411240125203001303220000300002157949889443151254523222 Sequence: ATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQT Prediction: -----+-+++++++-----------------+++++++++++++++-+++++-----+-- Confidence: 000014144565340200000000000102265567587765863335674632020401 Sequence: RTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARE Prediction: ------+++--------------++-+++++++++++++++-+++--++-++--+++-++ Confidence: 201122434320000000000024324456685767755561486336429522574366 Sequence: TKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQI Prediction: ++--+++++++------+-++-+++++++++++++-++---++-++++-++-++++-++- Confidence: 673358766632020003046058487695987783651334426655244146531440 Sequence: CNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACF Prediction: --++++++++++++++++--++++--+++++---++++++++++++++--++-+++++++ Confidence: 024458576464784754213766327665312157699977766864235516575497 Sequence: NHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKN Prediction: ++++-++-++++--+-+--+++++++++-+----------+-----+--+-+++++++++ Confidence: 665436434755335232245577634513321102230052222242343564355558 Sequence: AKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPN Prediction: +-+++++++++++++-+++++++-+++------+-++++-++-+--++---+--++---+ Confidence: 513433575786787347748842476310112334443346351254212431452133 Sequence: LPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYN Prediction: -+-+---------------------+----+++-+++-+-+++----+-------+++++ Confidence: 351423200100101000011000040021457365426165423225300101365654 Sequence: HNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEH Prediction: ++---+--+--+++---------++-+++--+-++--+--++--+--+--+--------- Confidence: 451026214235343010001103705543252561351344334025224100113001 Sequence: NPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDN Prediction: --------------+-+-++--+-+++-++--+--------------------------- Confidence: 000020000000213340341142444244204312002003103212001000000000 Sequence: DGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRF Prediction: ----+-+++-+---+-+++--++++--+-------------------------------- Confidence: 010041346291115246421545432300001000000000010000000000020322 Sequence: QMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDD Prediction: ----+++----------------------------------------------------- Confidence: 000145430231020320000001000101113100100000000000301000100100 Sequence: DYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNAL Prediction: --------++++++-----------------------+--------+++++++++--+-- Confidence: 010001204544650010000000002000000023033321120056669878503400 Sequence: WHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYD Prediction: +--++-----+--++--+--+++-+-+++-+-++++-----+-+-+-------------- Confidence: 330382220082334219216652415341503686300204030300000000000010 Sequence: KTYAGGRLGLFVFSQEMVFFSDLKYECRDP Prediction: ---++++-++---------------+--+- Confidence: 112335515420000000000001240233 *Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *Confidence: from level 0 (lowest) to level 9 (highest).

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