| Detail-Interactions: | |
| RAID ID: | RPI00001614 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | SERPINE1 |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-103 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and SERPINE1 | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | SERPINE1 Location |
| The Predicated Binding Sites between TERC and SERPINE1 | ||||
| By RIsearch | Structure | TERC Location | SERPINE1 Location | Score |
| The Predicated Binding Sites of SERPINE1 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P05121|PAI1_HUMAN Plasminogen activator inhibitor 1 OS=Homo sapiens GN=SERPINE1 PE=1 SV=1 Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPY Prediction: ----------------------------------------+------+-++-+------- Confidence: 958248727999999897968246223422742882678849735775257662755463 Sequence: GVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAI Prediction: ------------++++++---------+----------+-----------+---+----- Confidence: 576299969582655465238376576485537433777383379654544526424574 Sequence: FVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAV Prediction: --------------------+-+--+-------+-+----------++--------+--- Confidence: 695289278389978993977348258662273685699785622247356228867656 Sequence: DQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPD Prediction: ----+-----------------++++++++---+-+-++-+-----+-++--+-+-++-- Confidence: 448268989488362433222346867588352624266657554542573253464522 Sequence: GHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPK Prediction: -------------------------+-------+-----------+-+-++--+------ Confidence: 434599969734442727888663754866478438726378355436268737789873 Sequence: FSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASS Prediction: ---------+-----------------------------------+-+-------+-+++ Confidence: 647643667624752977356826957654254646785983772485626322372475 Sequence: STAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP Prediction: ++------+-------------------++-+---------- Confidence: 663547287676899999499778924256357889969978 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P05121|PAI1_HUMAN Plasminogen activator inhibitor 1 OS=Homo sapiens GN=SERPINE1 PE=1 SV=1 Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPY Prediction: ----------------------------++--+----------+---------+------ Confidence: 628469879999999999948373122234853565366518357943522626542333 Sequence: GVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAI Prediction: +-----------------------------------------------------+--+-- Confidence: 443276779274421222349889969598889764771355178463355566422366 Sequence: FVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAV Prediction: ---+-------------------------------------------------------- Confidence: 263527365495658965633669739251236592587163522451136428758866 Sequence: DQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPD Prediction: ------------+-+-++-+----------------------+-++-------------- Confidence: 648526887256334333242452121224651211142513414327214546387698 Sequence: GHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPK Prediction: ----------+------------------------------------------------- Confidence: 885878737243255567987853523678779989979778453641663577574622 Sequence: FSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASS Prediction: -----------------------------------------+--++-------------- Confidence: 716335778235862648215837538462274643682524445492558536428324 Sequence: STAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP Prediction: --------+--------------------+-------+--++ Confidence: 637778884777978374156278956234424635654276 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P05121|SERPINE1 Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPY Prediction: +++-+-------------------------------+------+---------+---+-- Confidence: 575472011000000000000000000002012131542030260012043125234544 Sequence: GVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAI Prediction: ++++-------+-+--+-----+--------------------------+----+--++- Confidence: 755542131227363450120051131200000011212101010133253302724964 Sequence: FVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAV Prediction: +--------------------------+-----------+-++--+-+-+---------- Confidence: 510421100001000102112110441532121103234527734736351440113211 Sequence: DQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPD Prediction: ---------+++++++++-++---++++-++-+-+++++++++++++--+-+-------- Confidence: 411234144867898696455443675826647357676888999874453602000000 Sequence: GHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPK Prediction: -------------+------------------------------+---------+-+-++ Confidence: 000020121211153030011001311110110021040131016123201010515378 Sequence: FSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASS Prediction: -+----------------+------+---++---+-----++--+--------+++++++ Confidence: 471201010111024012510211363438622153322056116313111316787887 Sequence: STAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP Prediction: --------+----++++++++++-++++++++++++++-+++- Confidence: 4421112153142667599965736899888758867946870 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPY Prediction: +-+---------------------++++++---------++--+-----++-+++----- Confidence: 323130000000000000000112336565102000000451330001059149300100 Sequence: GVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAI Prediction: ------------++++++------------------------------------+----- Confidence: 000000000032555974000000000000000023011300000100202202703200 Sequence: FVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAV Prediction: ----+---------------------++-+------++++++--++--+-------+--- Confidence: 003040000000000000000000014925301301753879109820530020003020 Sequence: DQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPD Prediction: -----------++++-+-+++++----++---+-+-+++-+++-+++----------+-- Confidence: 000000000005766052647943320360007262445064727641000000311301 Sequence: GHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPK Prediction: ---------++------------------------------------+---+--+++--- Confidence: 001020010850000000000000020100000000000000021114200430644021 Sequence: FSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASS Prediction: ---+-----------------------+-++----------+--++-------------- Confidence: 000500000000000000010000000603532220000039237800000000000000 Sequence: STAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP Prediction: --------+--+-----+++---------+++++++++-+++ Confidence: 000000014015020105960000010218735766391499 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.