| Detail-Interactions: | |
| RAID ID: | RPI00001611 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | NFKBIA |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-100 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and NFKBIA | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | NFKBIA Location |
| The Predicated Binding Sites between TERC and NFKBIA | ||||
| By RIsearch | Structure | TERC Location | NFKBIA Location | Score |
| The Predicated Binding Sites of NFKBIA | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P25963|IKBA_HUMAN NF-kappa-B inhibitor alpha OS=Homo sapiens GN=NFKBIA PE=1 SV=1 Sequence: MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVP Prediction: ------+---------+++++--+-------+-----+--------------+------- Confidence: 993764642447547385547245547634752322355554588948946858442253 Sequence: RGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVI Prediction: +-++--+---------------------------+------------------------- Confidence: 736622523724686379699998982772569965826678585854372234847888 Sequence: TNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATN Prediction: -------------------+--+-+-------------+----+-+-++-------+-+- Confidence: 345678969977654435864474424627888665774766652535622832685462 Sequence: YNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCG Prediction: ++---------------------------------+-++--------------------- Confidence: 654225738728464998979993986462633225475652989793465782989276 Sequence: ADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTE Prediction: ----+-++++++-+-----+++++-++---+--------------------+-------- Confidence: 536285566565252522385679556265483876968956454532253437745978 Sequence: DELPYDDCVFGGQRLTL Prediction: -------------+-+- Confidence: 76866988866536747 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P25963|IKBA_HUMAN NF-kappa-B inhibitor alpha OS=Homo sapiens GN=NFKBIA PE=1 SV=1 Sequence: MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVP Prediction: ------+---------+---+--+------------------------------------ Confidence: 222573313425465242134346458447945755648368659968947828956565 Sequence: RGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVI Prediction: ------------------------------------------------------------ Confidence: 232684477867366278296864745999999949977688673845462524728776 Sequence: TNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATN Prediction: ------------------------------------------------------------ Confidence: 574789959885477232713413528759887897999989897468676877874846 Sequence: YNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCG Prediction: ------------------------------------------------------------ Confidence: 748759759777774999979895689674853433531772879577589995989256 Sequence: ADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTE Prediction: ---+--+----------------------------------------------------- Confidence: 624333322332545967837357986887376964955857776967689889998989 Sequence: DELPYDDCVFGGQRLTL Prediction: ----------------- Confidence: 99998999664412524 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P25963|NFKBIA Sequence: MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVP Prediction: --+---++--------------------+------------------------------- Confidence: 005121562132010011000100000051231231101102100110010020100100 Sequence: RGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVI Prediction: --+-----------++-++-+------------------+--------+-++++++-+-+ Confidence: 117323020113447845535224120000000000002520011032545867764536 Sequence: TNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATN Prediction: +-+---------+++------++++++++++-++-+---------+-+------------ Confidence: 648341122324975422044677888875736525110000104645120120011021 Sequence: YNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCG Prediction: -+-+--+--+-++++--------+++++---+--++++++++----------------++ Confidence: 254644644645657241022217876614052467889875441211112002211376 Sequence: ADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTE Prediction: --------+++++++-+-++-+-+-------++--------------------------- Confidence: 441324345577656162574626012241475101000000000000000000000000 Sequence: DELPYDDCVFGGQRLTL Prediction: ------------++---- Confidence: 000000001243873330 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVP Prediction: --+++-+---+---+++-+-+--++------++-------------------+---+--+ Confidence: 216432432030104542505304300120243002000011002000010040334235 Sequence: RGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVI Prediction: -----++-+-----+-+-+-+-+---------------------+--------+++---- Confidence: 201034424100316252924032230000000000002010003000000106590003 Sequence: TNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATN Prediction: -----------+----+---+-+-+++-+--------------------+---------+ Confidence: 010021030005201050223230437180000001001000002210141010102123 Sequence: YNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCG Prediction: -----+-+---------------+++++++----+-++++-+---------------+++ Confidence: 122224280002002000000014645483312273578739000100000100001786 Sequence: ADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTE Prediction: +-+-----+---+-+--------------------------------------------- Confidence: 536110204323405000100210103000010001110122000000000000000000 Sequence: DELPYDDCVFGGQRLTL Prediction: -------------+--- Confidence: 00010000000213111 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.