| Detail-Interactions: | |
| RAID ID: | RPI00001609 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | MYC |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-98 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and MYC | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | MYC Location |
| The Predicated Binding Sites between TERC and MYC | ||||
| By RIsearch | Structure | TERC Location | MYC Location | Score |
| The Predicated Binding Sites of MYC | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P01106|MYC_HUMAN Myc proto-oncogene protein OS=Homo sapiens GN=MYC PE=1 SV=1 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPP Prediction: -----+-+++-+------------------------+----------------------+ Confidence: 869375365634354363735484889965723222422622263224643697674244 Sequence: LSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPD Prediction: -++++++++-+--+--------+--+-+++-+-+-------------------------- Confidence: 287688864253257862252585242455252526748789589987778468897588 Sequence: DETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQD Prediction: ------------------+---+----+--+++-++++++++++++-++--++++----- Confidence: 876793599989996676455465625446568249778698865946722566563747 Sequence: LSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVL Prediction: ---------------------+++++++-+--++-++++--+--++++++++++------ Confidence: 926662478776997635722678698426236634665236366575787657454899 Sequence: HEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRC Prediction: ---+-++++++++---------------+++++++++++++++++++++++++-----+- Confidence: 785526577765524378898979625258859799999786878688965652667264 Sequence: HVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHN Prediction: +-+++--++--++++++++++-++-+----+--+--++++++++++++-++++-+++--+ Confidence: 546642345326789975974378382325585623456897986967256663777227 Sequence: VLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLL Prediction: +--++++++-++----------------+--+-----+-+-+------------------ Confidence: 522659887277435582969648434464366778255555782726454378587544 Sequence: RKRREQLKHKLEQLRNSCA Prediction: ++++++-+------+---- Confidence: 7588782732652864357 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P01106|MYC_HUMAN Myc proto-oncogene protein OS=Homo sapiens GN=MYC PE=1 SV=1 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPP Prediction: -----+--++-----------------------------------++--++--------+ Confidence: 327253333622555566827685989888856673454632452541154264454234 Sequence: LSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPD Prediction: ++++++------------------------------------------------------ Confidence: 487884115466857777464514526646767689899999899978787487699887 Sequence: DETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQD Prediction: --------------+---+---+----+--+++--+-++++++++++++--+++------ Confidence: 784581236623554233415545535533337226134344644634422434123888 Sequence: LSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVL Prediction: ---------------------+-+++---------------------------------- Confidence: 747875856365756752512415451211232265575978898788688778999999 Sequence: HEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRC Prediction: ----------------------------++--+++++++++-++-++++-+-------+- Confidence: 999999999999999999999989825245165377548532443335613132325143 Sequence: HVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHN Prediction: +-+-+++++------+---++-++-+--------+-++++++++++++-+----+++-++ Confidence: 425355434343531325333364245535283242344784676834231224486277 Sequence: VLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLL Prediction: ++-++++++-+-----------------+--+----++---------------------- Confidence: 341858875443667692889357333372153157445382743625572256575655 Sequence: RKRREQLKHKLEQLRNSCA Prediction: ++-------------+-+- Confidence: 6332725345872713434 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P01106|MYC Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPP Prediction: -----+----------------------------------------------------++ Confidence: 131215203411100010000100000000000000000011421330031001011455 Sequence: LSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPD Prediction: +++++------------------------------------------------------- Confidence: 677752110100100000000000000000000000000000000000000000000000 Sequence: DETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQD Prediction: ----------+++++++++++-+----+--+++-+++--+----------+--------- Confidence: 000101101066556988867352144524558478613532241232117324314100 Sequence: LSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVL Prediction: ----------+-----++------------------------------------------ Confidence: 020000210254234265042301100000000100000000000000000000000000 Sequence: HEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRC Prediction: --------------------------------------------------+------++- Confidence: 000000000000000000000000010122111132202001110132435232112552 Sequence: HVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHN Prediction: ----+--++++++------+--++-+-+--+--+++-++++++++++++-+-+-++++++ Confidence: 202363488656522220352167152533820695498999899976745374887788 Sequence: VLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLL Prediction: --+++++++-++-+-+-+-------++-+--+----++--+----------+-------- Confidence: 436889986376453616211220165383363344881352112110134500112242 Sequence: RKRREQLKHKLEQLRNSCA Prediction: --+++++-++-++-+++++- Confidence: 44875754762584778870 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPP Prediction: --+---+--+-+------------------------------------------------ Confidence: 233223411523200010000000000000000000000000001221000000001031 Sequence: LSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPD Prediction: ++--+-++---------------------------------------------------- Confidence: 361143533312211001100121011101202100000000000000000000000000 Sequence: DETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQD Prediction: ------++------++++--+------+++-+++----++--++---------+------ Confidence: 000002340000126575323021012443144423123422441322323223211010 Sequence: LSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVL Prediction: -----------------+--++-------------------------------------- Confidence: 101000000000000125234531010200000000000000000000000000000000 Sequence: HEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRC Prediction: ---------------------------+++++++++++++-++---+------+---++- Confidence: 000000000000000000000000001699878869555533530233212304303533 Sequence: HVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHN Prediction: --+-+-+-+++++-+-------++-+++-+++++++++++++++++++++++++++++++ Confidence: 135332637447524200020369253523645734498899999989945566579889 Sequence: VLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLL Prediction: +++++++---++----------------+--+-+---+-------------+-------- Confidence: 858995512254001002000000010363243511230111110200322410101022 Sequence: RKRREQLKHKLEQLRNSCA Prediction: -++++++-++----+++++ Confidence: 2475343166223367773 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.