| Detail-Interactions: | |
| RAID ID: | RPI00001606 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | JUN |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-95 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and JUN | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | JUN Location |
| The Predicated Binding Sites between TERC and JUN | ||||
| By RIsearch | Structure | TERC Location | JUN Location | Score |
| The Predicated Binding Sites of JUN | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P05412|JUN_HUMAN Transcription factor AP-1 OS=Homo sapiens GN=JUN PE=1 SV=2 Sequence: MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDL Prediction: ---+--+--------------++-++++++++--+------------+-+++-+-++++- Confidence: 625563528557894833532552775764474663353649965646474648465654 Sequence: LTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAE Prediction: -+-------+--+----+-----++++-+++++----------------------+---- Confidence: 543675567464533866768226548376856222476533726666869596959883 Sequence: LHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGA Prediction: -----+--+-++-----+----------+--+++++++-+-+-------+---------- Confidence: 722225325656352365565577488764344759652626532334758262263234 Sequence: LSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETP Prediction: -+++++-+++---------+-+++++--------------+-+--+-------------- Confidence: 477567257635557643552665642324754574722476444564343734535323 Sequence: PLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANM Prediction: -----------+-+-++++++++--++++++++-++--+---+-++-+-++++--++-+- Confidence: 263364432336382799959583368959898468575743556638354654355353 Sequence: LREQVAQLKQKVMNHVNSGCQLMLTQQLQTF Prediction: -+---------------+------------- Confidence: 6586862922286248355626683438327 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P05412|JUN_HUMAN Transcription factor AP-1 OS=Homo sapiens GN=JUN PE=1 SV=2 Sequence: MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDL Prediction: ---+------------------------+--+--+--------------+++-+------ Confidence: 213465478477784754643642335232262352262747963511253355435766 Sequence: LTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAE Prediction: ---------+---------------------++--+------------------------ Confidence: 753543376454163973999525444855335215386423515557979887949684 Sequence: LHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGA Prediction: --+--------------+--+-------------+++----------------------- Confidence: 416222654732442234233434245672464235321142545665656286343324 Sequence: LSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETP Prediction: ---------+---------++-++++--++----------+-+---------+------- Confidence: 322534552434224312354154432243343243412153523153223444431212 Sequence: PLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANM Prediction: ++++---------+--+++++++-+++++++++--+------------------------ Confidence: 556323575541453179848882457849598135645864598582654868678947 Sequence: LREQVAQLKQKVMNHVNSGCQLMLTQQLQTF Prediction: ----------------------+---+-+-- Confidence: 9188582925667326233518385233342 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P05412|JUN Sequence: MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDL Prediction: -----------------------------------+------------------------ Confidence: 113110010000000000000000000000140025113110211040032102000010 Sequence: LTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAE Prediction: ------------------------------------------------------------ Confidence: 001000100001013002000000000002110112000100000000000000000000 Sequence: LHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGA Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000001000010001000100000000000000000000012 Sequence: LSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETP Prediction: ------------------------------------------------------------ Confidence: 131222111221111111121110011011020000000000000000000000000000 Sequence: PLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANM Prediction: -----------+-++-++++++++++++++++++++--+--------------------- Confidence: 002100010335467476657997798899799769438310300000012001100012 Sequence: LREQVAQLKQKVMNHVNSGCQLMLTQQLQTF Prediction: ------+-------------+-+--++-+--- Confidence: 03121171331103312434627348535420 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDL Prediction: -----------------------++----------------------+-++++++----- Confidence: 201000000000000000021104321000020000000011200233044344432000 Sequence: LTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAE Prediction: ---------+--++---+-----------------------------------------+ Confidence: 012322221412330004000121100000003202000230000000000001000004 Sequence: LHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGA Prediction: -------------------------------------------------------+---- Confidence: 000100012121000000000000000000000001000001000100000003140122 Sequence: LSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETP Prediction: -------------------------------------------------------+++-+ Confidence: 121112111000000000010000112112201201000000000000000030033337 Sequence: PLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANM Prediction: -+-+-------++++++++++++++++++++++++++-++--+---++--+-+------- Confidence: 341422010205645487989998999999999569719720300254135341111011 Sequence: LREQVAQLKQKVMNHVNSGCQLMLTQQLQTF Prediction: --------------+++++------------ Confidence: 0121000001210255546100100010011 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.