| Detail-Interactions: | |
| RAID ID: | RPI00001602 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | BIRC5 |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-91 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and BIRC5 | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | BIRC5 Location |
| The Predicated Binding Sites between TERC and BIRC5 | ||||
| By RIsearch | Structure | TERC Location | BIRC5 Location | Score |
| The Predicated Binding Sites of BIRC5 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|O15392|BIRC5_HUMAN Baculoviral IAP repeat-containing protein 5 OS=Homo sapiens GN=BIRC5 PE=1 SV=3 Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFC Prediction: -----------------+--+------------+--+----------------------- Confidence: 666425348447693756624536588988765445567488573542546578959899 Sequence: FKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNK Prediction: -----------------+++++-------+------------+--+++-+++--++++++ Confidence: 958967467788674526566633536274237559598676562758287634868878 Sequence: KKEFEETAKKVRRAIEQLAAMD Prediction: ++------++-++--------- Confidence: 7623233456688686499988 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|O15392|BIRC5_HUMAN Baculoviral IAP repeat-containing protein 5 OS=Homo sapiens GN=BIRC5 PE=1 SV=3 Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFC Prediction: -----------------+------------------------------------------ Confidence: 226225234378365753861226347888887468678376674542226647557763 Sequence: FKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNK Prediction: ----------------+-+--+-----------------------------------+-- Confidence: 465627286566123341412325867563246869798788497553623467283345 Sequence: KKEFEETAKKVRRAIEQLAAMD Prediction: ---------------------- Confidence: 5474865724722496577788 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>O15392|BIRC5 Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFC Prediction: --------------------++--+---+-+--+--+---+++-+--------+-++--- Confidence: 122211222120000004035544842254531544722457727212244335375233 Sequence: FKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNK Prediction: --+-+--+--------+-++++++-+---------------------------------- Confidence: 346254452123323454986667262222411000000200000001000200200000 Sequence: KKEFEETAKKVRRAIEQLAAMD Prediction: ----------------------- Confidence: 00000000000000001012110 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNI Prediction: --++-+--+------------+------+----------------------------++- Confidence: 213534225001100000000401211243000000000000000000222132123441 Sequence: HVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILK Prediction: -------------------------------+++-------------------------- Confidence: 200000000010001000000000000002034430200020102110020000000000 Sequence: MLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLG Prediction: -------++---+----------------------------------------------- Confidence: 003022343122501131000000110000000010000000000012022211100101 Sequence: CKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVE Prediction: ------------------------------------------------------------ Confidence: 210021210000000000000000100002002220000000000010000000000000 Sequence: VLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQ Prediction: -------+---------+--------------+-------+------------------- Confidence: 100013232220000023012121100000024001000041000000030100000000 Sequence: QYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWH Prediction: --------------------------------------+-------------------++ Confidence: 001021000011000000000000000000011133124012000000002000000233 Sequence: RKs194359942394369194475953336294354153437196822236451128323 Prediction: ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 744444444444444444444444444444444444444444444444444444444444 Sequence: 256585643882964416492675665191688719895341945734645451119764 Prediction: ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 444444444444444444444444444444444444444444444444444444444444 Sequence: 497789213177875855933974262131133119977996699993322446644775 Prediction: ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 444444444444444444444444444444444444444444444444444444444444 Sequence: 55511991144228 Prediction: ++++++++++++++ Confidence: 44444444444444 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.