| Detail-Interactions: | |
| RAID ID: | RPI00001601 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | BCL2L1 |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-90 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and BCL2L1 | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | BCL2L1 Location |
| The Predicated Binding Sites between TERC and BCL2L1 | ||||
| By RIsearch | Structure | TERC Location | BCL2L1 Location | Score |
| The Predicated Binding Sites of BCL2L1 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|Q07817|B2CL1_HUMAN Bcl-2-like protein 1 OS=Homo sapiens GN=BCL2L1 PE=1 SV=1 Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLA Prediction: -+++-+---------+-+++++++---------++--++---++-------+-+------ Confidence: 466526599999833556777656347336232552255623544433244525234468 Sequence: DSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAY Prediction: -----+-++-++++---+--------+---+----------++--+-----------+-+ Confidence: 544437358287773475697669786257757776737237762537232746224546 Sequence: QSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEP Prediction: -----------+------+-------------------------+--------------- Confidence: 238759957985767448798889578778683377278488835896489339576988 Sequence: WIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK Prediction: -------------------+-++++++++--+---------------+--+++ Confidence: 69665664767979373335265986878446687389387689896444475 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q07817|B2CL1_HUMAN Bcl-2-like protein 1 OS=Homo sapiens GN=BCL2L1 PE=1 SV=1 Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLA Prediction: -++--+---------+-+++---------------------------------------- Confidence: 345124779894611834542382899989786457526645455767365253221267 Sequence: DSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAY Prediction: ------------------------------+-----------+----------------- Confidence: 744341532453667795786789665478358877543224486789983959467484 Sequence: QSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEP Prediction: ---------------++-+----------------------------------------- Confidence: 766769998982635642654587337568663586479799984899899968663544 Sequence: WIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK Prediction: +------+---------------+----+----------------------++ Confidence: 53322334466978684567938526123452587696576588684334178 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q07817|BCL2L1 Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLA Prediction: ----++--------++-+++---------------------------------------- Confidence: 143465210002126837674413200000000000000000000000000000000000 Sequence: DSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAY Prediction: -------------------------------+---------------------------- Confidence: 000000000000000000003122213331250011011122200110013010101202 Sequence: QSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEP Prediction: ---------------++++----------------------------------------- Confidence: 000000002001133876833142242320011000111010014000100000001011 Sequence: WIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK Prediction: +-+++++++-------------------+----------------------++- Confidence: 526798877410100011111102324264444321111211211103100850 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLA Prediction: +++---------+-++--+-++-------------------------------------- Confidence: 443123021001723803524303010100000000000000000000000001011110 Sequence: DSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAY Prediction: --+------------------------+-------------------------------- Confidence: 324000100000000000000010013400310000001331100000000000100003 Sequence: QSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEP Prediction: -----------++++-+-+---+--+-------------------------+-------- Confidence: 000010000125855132400031040000003000000000000000000300011101 Sequence: WIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK Prediction: ---+++--------------------++-+++-------------------++ Confidence: 31157612002000000001221211332344211301200000000000165 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.