| Detail-Interactions: | |
| RAID ID: | RPI00001600 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | BCL2 |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-89 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and BCL2 | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | BCL2 Location |
| The Predicated Binding Sites between TERC and BCL2 | ||||
| By RIsearch | Structure | TERC Location | BCL2 Location | Score |
| The Predicated Binding Sites of BCL2 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>BCL2 Sequence: MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPA Prediction: -----++++--+---------+-+++-+---------------------++++-++-++- Confidence: 772529887325687734842645673445688584452233426236445742762662 Sequence: ASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLH Prediction: -++-+--++++-++---+--------+----------+------++++++---------- Confidence: 357262585864662444444322565495589393964642226776784344732274 Sequence: LTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEY Prediction: ----+-+-+----------------+-----------------+---------------- Confidence: 633454837582996698286744879998999989778326365626998699979465 Sequence: LNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK Prediction: --+-----------------------+-----------------------+---+---- Confidence: 94548333965576489699924426658999859492388289896879566655522 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P10415|BCL2_HUMAN Apoptosis regulator Bcl-2 OS=Homo sapiens GN=BCL2 PE=1 SV=2 Sequence: MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPA Prediction: +-+--++++-++-------+++-+++---------------------------------- Confidence: 325316766235688872534827762273788884774667889989854354114225 Sequence: ASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLH Prediction: ------------------------------------------------------------ Confidence: 566989958858565662263344674467887476935888863133428767997397 Sequence: LTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEY Prediction: ----------------------++-+---------------------------------- Confidence: 964849385696999898263474265457734755778349727868999599999795 Sequence: LNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK Prediction: -------+------+---------+--------------------------------++ Confidence: 77546454433233447798856332258665527193385177496668464513366 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P10415|BCL2 Sequence: MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPA Prediction: --+---+++++--------+++++++++-------------------------------- Confidence: 216144557783321014368989785602100100000000000000000000000000 Sequence: ASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLH Prediction: --------------------------------------+--------------------- Confidence: 000000031100000000000000000101130342127011102111010001000300 Sequence: LTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEY Prediction: ------+---------------++++---+------------------------------ Confidence: 011130611000001200213477682315244343000100001100001401010000 Sequence: LNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK Prediction: -------+-+++++++--------------+--+++-+---------------++--++- Confidence: 001202374889997642011002112101544865162212322222122135524980 Sequence: Prediction: Confidence: *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPA Prediction: --+-+++++--+------+--+-+++++-------------------------+++---- Confidence: 113358754014000000723613656500000000000000000000000225342122 Sequence: ASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLH Prediction: -------------------------+---------------------------------- Confidence: 000000000011102132323111031000001010032011000122110000000001 Sequence: LTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEY Prediction: ------+-----------++++-+-+------+--------------------------- Confidence: 010010301001000001475514240003205000001200000000000000000020 Sequence: LNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK Prediction: -------+--+++----+------------+-+++++-+------------------++ Confidence: 00021024115772210300010110121342444442300000000000000000076 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.