| Detail-Interactions: | |
| RAID ID: | RPI00001599 |
|---|---|
| RNA/Protein Symbol 1: | TERC(htr) |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | BAX |
| RNA/Protein Category 2: | Protein |
| Validated Method: | quantitative fluorescent reverse transcription polymerase chain reaction |
| Tissue: | bladder cancer cell line biu-88 |
| PMID: | 20556588 |
| Detail description: | The effects of combined RNA interference (RNAi) of human telomerase RNA (hTR) and human telomerase reverse transcriptase (hTERT) genes on telomerase activity in a bladder cancer cell line (BIU-87 cells) were investigated by using gene chip technology in vitro with an attempt to evaluate the role of RNAi in the gene therapy of bladder transitional cell cancer (BTCC). HTR-siRNA and hTERT-siRNA, especially their combination, siRNA hTR+hTERT, specifically and effectively suppressed the expression of both hTR and hTERT mRNA and telomerase activity. Gene chip analysis revealed that 21 genes were down-regulated (ATM, BAX, BCL2, BCL2L1, BIRC5, CD44, CTNNB1, E2F1, JUN, MCAM, MTA1, MYC, NFKB1, NFKBIA, NME4, PNN, PNN, SERPINE1, THBS1, TNFRSF1A, and UCC1). |
| The Predicated Binding Sites between TERC and BAX | |||||
| By miRanda | Structure | Match Score | Energy Score | TERC Location | BAX Location |
| The Predicated Binding Sites between TERC and BAX | ||||
| By RIsearch | Structure | TERC Location | BAX Location | Score |
| The Predicated Binding Sites of BAX | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|Q07812|BAX_HUMAN Apoptosis regulator BAX OS=Homo sapiens GN=BAX PE=1 SV=1 Sequence: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLS Prediction: ---+-+++++++++++-+--+------------+--+-----------------++++-+ Confidence: 754625669688787626555467779486956653932238559887674332766656 Sequence: ECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKL Prediction: ----+-------------------+++-+-------------------+----------- Confidence: 258259954872375752996474646463879399878826735327599899589329 Sequence: VLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGV Prediction: --+----------+----------+---------------------+------------- Confidence: 975767229548954767568769494996496657646668325563232294989878 Sequence: LTASLTIWKKMG Prediction: -+-+-------- Confidence: 867452842267 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q07812|BAX_HUMAN Apoptosis regulator BAX OS=Homo sapiens GN=BAX PE=1 SV=1 Sequence: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLS Prediction: ---+--+-+-++-------------------+-+--+----------------------- Confidence: 332433415133336385896999999795835344344444467788785234134353 Sequence: ECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKL Prediction: -----------+-------------------------------+-++-+----------- Confidence: 357349978843455433888998747827888599989838432742554587347438 Sequence: VLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGV Prediction: ------------------------------+------+---------++-+--------- Confidence: 765775659879987879897978674687374323343689554624413587979888 Sequence: LTASLTIWKKMG Prediction: ---------+-- Confidence: 947484741432 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q07812|BAX Sequence: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLS Prediction: --------------------------------++-++-+---+---------++------ Confidence: 000000012100100000000000000112245626647304610110123365342311 Sequence: ECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKL Prediction: -------------------------------------------+-++++-+-+-++-++- Confidence: 200020101011001000100100000022001200020010153889946452563551 Sequence: VLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGV Prediction: ------------------------------+-++++++---------++-+--------- Confidence: 012000111010013000100000111013735798854420122445636112001010 Sequence: LTASLTIWKKMG Prediction: --------++-+- Confidence: 0101111366250 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLS Prediction: +-+-----+-++-+-------------------+---+---------------------- Confidence: 434321235244333020001000010011020302333210000000000110010003 Sequence: ECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKL Prediction: -------------+--++-----------------------------++----------- Confidence: 200210000102242154121000010121200000000000110204502021020000 Sequence: VLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGV Prediction: ---------------------------------+++------------------------ Confidence: 002010000000000000000200001000213356111010000002101000000010 Sequence: LTASLTIWKKMG Prediction: ------------ Confidence: 000000000000 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.