| Detail-Interactions: | |
| RAID ID: | RPI00001597 |
|---|---|
| RNA/Protein Symbol 1: | MBII-52 |
| RNA/Protein Category 1: | snoRNA |
| RNA/Protein Symbol 2: | NCL(Nucleolin) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | RNP Immunoprecipitation, Mass spectrometry |
| Tissue: | |
| PMID: | 20484469 |
| Detail description: | Among these, Nucleolin and ELAVL1 proteins were confirmed to independently and directly interact with the MBII-52 snoRNA by coimmunoprecipitation. |
| The Predicated Binding Sites between MBII-52 and NCL | |||||
| By miRanda | Structure | Match Score | Energy Score | MBII-52 Location | NCL Location |
| The Predicated Binding Sites between MBII-52 and NCL | ||||
| By RIsearch | Structure | MBII-52 Location | NCL Location | Score |
| The Predicated Binding Sites of NCL | |
| RBPBD | RRMx4 |
| RsiteDB | |
| BindN |
>sp|P19338|NUCL_HUMAN Nucleolin OS=Homo sapiens GN=NCL PE=1 SV=3 Sequence: MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATS Prediction: -----+-+++++++++--+--+----------------------------++++++++++ Confidence: 892748269897756622534546356567556558544532654778527959965577 Sequence: AKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVA Prediction: -++---+++++----++-++---++-++--+++-+++-+--+--++++++++++-+---- Confidence: 477544545663453654783327628822587287636237338884996795374554 Sequence: TPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAA Prediction: ++-++--+---++++++++++++-+------------------------------+---- Confidence: 762882344238559889959852524745664456558778778889897686657554 Sequence: APASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEE Prediction: ---+-----------------------------+++++++--+----+-+---------- Confidence: 424535556787777788778666656856743574959932846276862764777677 Sequence: DDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEG Prediction: ---------------------------------+--++++++++-+----++-++++--- Confidence: 777778777777777777777777777887868432868989862722527729757322 Sequence: TEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDL Prediction: ---+-------------++--+-++----------------+----++--+--------- Confidence: 223627639798272426532467456227683898879796642275557757648789 Sequence: EKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIR Prediction: ---------+------+-+++++++++++++-+----+---++----------------+ Confidence: 637967357574744755575969999869638255662535572277938999998695 Sequence: LVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNST Prediction: --+++++++---+----------+----+--++---+--+--++-+++++++++++++++ Confidence: 555885978346585734463434245242646433624752542585877579679867 Sequence: WSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEAL Prediction: +++++++------+++-+--------------+---+++++++-+--------------- Confidence: 776655557532655535364967988683586632598798826779799477882775 Sequence: NSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARI Prediction: ++-++++---+--+---+--+++++++++++++---+-----+---+-------+-+-+- Confidence: 563657542365596345229699969898886645555324422446253552657474 Sequence: VTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGR Prediction: ---+++++++-------------+--+-----------+-+----+-+---++-++++++ Confidence: 724657688843648674354646656476688484424646425735322552597689 Sequence: GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE Prediction: +++++++++++++++++++-++++-+++++++++++++++++++++++-- Confidence: 57746978996896886792957624955696695777679796997843 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P19338|NUCL_HUMAN Nucleolin OS=Homo sapiens GN=NCL PE=1 SV=3 |
| RNAbindR |
>P19338|NCL Sequence: MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATS Prediction: --+++++++++++-++-------------------------------------------- Confidence: 439589768987647613121100000000000000000000000000000000000000 Sequence: AKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVA Prediction: ------------------------------------------------------------ Confidence: 000000000110000021311101112100010000001000000000000000000000 Sequence: TPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAA Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000000000000000000000000 Sequence: APASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEE Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000100000001111101000000 Sequence: DDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEG Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000011244431010000000001000001 Sequence: TEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDL Prediction: ------------+--+-----------------------+-++++++++++-+--+---- Confidence: 000101041314613630000000000000001013413548789999889373351010 Sequence: EKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIR Prediction: -------------+-++-++++++++++++-+-+++-++--+------------------ Confidence: 021011301323483592678878586666452967387345301201020010101212 Sequence: LVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNST Prediction: ----+++++++++----------------------++--+-------------------- Confidence: 124368889887823012000001002302111125722511323331300222120111 Sequence: WSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEAL Prediction: ------+-+-++------------------------+++++++++++-+--+-------- Confidence: 001221635267124201001000000001012114558798989873511530100000 Sequence: NSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARI Prediction: ---------++--+-++++++++++++---+-+-+-++------------------+-+- Confidence: 011412423574483678688687757444527464651220001010200011115372 Sequence: VTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGR Prediction: --++++++++++++----------------------+++-+---++++-+++++++++++ Confidence: 345668898998684101401101000100101122586374337577488568776888 Sequence: GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE Prediction: ++++++++++++++++++++++++++++++++++++++++++++++++--- Confidence: 588776567776565758877855878676999988785785767857440 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATS Prediction: --+++++++++++-+++-++--------------------------+---++-++--+++ Confidence: 225478879964415663431010000000000000000020000050325624633555 Sequence: AKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVA Prediction: ----------+-----+--+------++--+++-++------------++--+--+++-+ Confidence: 213000223342321140142013327522444253001003200112442333264525 Sequence: TPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAA Prediction: ++++++++-+-+++++++++---------------------------------------- Confidence: 365655353736848974652010000000000000000000000000000000110100 Sequence: APASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEE Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000100000000000000010000000000 Sequence: DDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEG Prediction: ---------------------------------+++++++++-+++++++--++------ Confidence: 000000000000000000000000000000020464346664334657731134311030 Sequence: TEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDL Prediction: -+++---+-+-+++++-----------------++-+++++++++++++-+++------- Confidence: 354511042918984710000000000000001462777799749998909580100000 Sequence: EKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIR Prediction: --------++---+-++-++++++++++++++-+++-+++-+-----------------+ Confidence: 000000004500371492999999789976541979299927100000000000000004 Sequence: LVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNST Prediction: -++++++++++-+++---------------------+--+++++++++++++++++++++ Confidence: 286779989990948000000000001000011002523649999998787678534546 Sequence: WSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEAL Prediction: +++-+-+-+-+++-++----------------+++++++++++++-+-+----------- Confidence: 976340606099909400000000000000007676796999999193900000000000 Sequence: NSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARI Prediction: ---------+++-+-+++++++++++-----++-+-+++-++--------------+-++ Confidence: 000003200474351999999965543222258360669164000000000000004167 Sequence: VTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGR Prediction: ++++++++++++-+-+----------------++--++-++-++++++++++++++++++ Confidence: 675875989999091913000001000100004522783491999999799999999999 Sequence: GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE Prediction: +++++++++++++++++++++++++++++++++++++++++----+-+-+ Confidence: 99999999999999999997989969999989899999866202231423 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.