| Detail-Interactions: | |
| RAID ID: | RPI00000638 |
|---|---|
| RNA/Protein Symbol 1: | MIR221(miR-221) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | ZFP36(TTP) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Luciferase Reporter Assay, RNP Immunoprecipitation, Immunoblotting |
| Tissue: | THP-1 cells, Log-phase cells |
| PMID: | 20435889 |
| Detail description: | The process required to disrupt protein synthesis followed Toll-like receptor 4 (TLR4)-dependent induction of microRNA (miR)-221, miR-579, and miR-125b, which coupled with RNA-binding proteins TTP, AUF1, and TIAR at the 3`-untranslated region to arrest protein synthesis. TTP and AUF1 proteins linked to miR-221, whereas TIAR coupled with miR-579 and miR-125b. |
| The Predicated Binding Sites between MIR221 and ZFP36 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR221 Location | ZFP36 Location |
| The Predicated Binding Sites between MIR221 and ZFP36 | ||||
| By RIsearch | Structure | MIR221 Location | ZFP36 Location | Score |
| The Predicated Binding Sites of ZFP36 | |
| RBPBD | Znf_CCCHx2 |
| RsiteDB | |
| BindN |
>sp|P26651|TTP_HUMAN Tristetraprolin OS=Homo sapiens GN=ZFP36 PE=1 SV=1 Sequence: MDLTAIYESLLSLSPDVPVPSDHGGTESSPGWGSSGPWSLSPSDSSPSGVTSRLPGRSTS Prediction: --------------------+-++++++++++-++-+++-+++++++++-++++++++++ Confidence: 999589675793746663524265565686572872565464566678826795778565 Sequence: LVEGRSCGWVPPPPGFAPLAPRLGPELSPSPTSPTATSTTPSRYKTELCRTFSESGRCRY Prediction: +---+---+------------+-----++++++++++++++++++++--++-+++++-++ Confidence: 532562335744655463674664235745788887799989966854275357769398 Sequence: GAKCQFAHGLGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHNPSEDLAAPGHPPV Prediction: --+----------++-++++++++----------+--+-++------------------- Confidence: 236535848674787279888664265338262473282676335256465665473225 Sequence: LRQSISFSGLPSGRRTSPPPPGLAGPSLSSSSFSPSSSPPPPGDLPLSPSAFSAAPGTPL Prediction: -+-+---+--++++++++++------+-+++++++++++++--------+--+----++- Confidence: 764472343269799886552333227378775877787652436272246553434872 Sequence: ARRDPTPVCCPSCRRATPISVWGPLGGLVRTPSVQSLGSDPDEYASSGSSLGGSDSPVFE Prediction: -++--+-----+-++-+------------++++-++--+----+-+++++++++-+---- Confidence: 396227263226378262639475764358657586336234243564785665253875 Sequence: AGVFAPPQPVAAPRRLPIFNRISVSE Prediction: -------------++----------- Confidence: 98967464433446653466293834 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P26651|TTP_HUMAN Tristetraprolin OS=Homo sapiens GN=ZFP36 PE=1 SV=1 Sequence: MDLTAIYESLLSLSPDVPVPSDHGGTESSPGWGSSGPWSLSPSDSSPSGVTSRLPGRSTS Prediction: ---------------------------+------+--------------------++--- Confidence: 667468874864744772551222333434524152422757765743567316434213 Sequence: LVEGRSCGWVPPPPGFAPLAPRLGPELSPSPTSPTATSTTPSRYKTELCRTFSESGRCRY Prediction: -----------+--------------------++-+---+++++++------------++ Confidence: 577822351344322333854367765334114424213656754315524544512154 Sequence: GAKCQFAHGLGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHNPSEDLAAPGHPPV Prediction: -------------+--+++++++-----------+-++--+------------------- Confidence: 211523623455352258687752244235447441542246336247667788345233 Sequence: LRQSISFSGLPSGRRTSPPPPGLAGPSLSSSSFSPSSSPPPPGDLPLSPSAFSAAPGTPL Prediction: -+---+------++--++----------------------------------------+- Confidence: 241233214531351245111122422523366553234333645663125534326264 Sequence: ARRDPTPVCCPSCRRATPISVWGPLGGLVRTPSVQSLGSDPDEYASSGSSLGGSDSPVFE Prediction: -+++-+-----------------------+-+---------------------------- Confidence: 345524357643411344314243652243233746742343667445312234534738 Sequence: AGVFAPPQPVAAPRRLPIFNRISVSE Prediction: --------++---++-----+-+-+- Confidence: 96855234432127331335473542 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P26651|ZFP36 Sequence: MDLTAIYESLLSLSPDVPVPSDHGGTESSPGWGSSGPWSLSPSDSSPSGVTSRLPGRSTS Prediction: ------------------------------------------------------------ Confidence: 010100000000000000021113100010000001000000000000000020002000 Sequence: LVEGRSCGWVPPPPGFAPLAPRLGPELSPSPTSPTATSTTPSRYKTELCRTFSESGRCRY Prediction: ---------------------------------------+++++++++++++++++++++ Confidence: 000010000001121111000000000100110010122577898886786867888898 Sequence: GAKCQFAHGLGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHNPSEDLAAPGHPPV Prediction: +++++++++-++-++++++++++++++++++++++++++++++++++--+------+++- Confidence: 799998656255385589999999988997777978898999976551271223245553 Sequence: LRQSISFSGLPSGRRTSPPPPGLAGPSLSSSSFSPSSSPPPPGDLPLSPSAFSAAPGTPL Prediction: -++--------------------------------------------------------- Confidence: 365211011111010000000000000000000000000020000000011010221230 Sequence: ARRDPTPVCCPSCRRATPISVWGPLGGLVRTPSVQSLGSDPDEYASSGSSLGGSDSPVFE Prediction: ------------------------------------------------------------ Confidence: 222111000000011001000001011000000000000000000000000000000000 Sequence: AGVFAPPQPVAAPRRLPIFNRISVSE Prediction: --------------+-+--++--+-+- Confidence: 000000000102436262467245450 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MDLTAIYESLLSLSPDVPVPSDHGGTESSPGWGSSGPWSLSPSDSSPSGVTSRLPGRSTS Prediction: ---------------------+-+-++++-----------------------+--+++++ Confidence: 211210120011032300322334234542220021000100001311121141347463 Sequence: LVEGRSCGWVPPPPGFAPLAPRLGPELSPSPTSPTATSTTPSRYKTELCRTFSESGRCRY Prediction: +----------+---------+-+-----+++++-+-+-+++----+-+-----+++-++ Confidence: 301210100024102021010314122225443525352354322343330330654356 Sequence: GAKCQFAHGLGELRQANRHPKYKTELCHKFYLQGRCPYGSRCHFIHNPSEDLAAPGHPPV Prediction: ++++++++++++++--++++++++++++------+-++++++++-+----------+-+- Confidence: 636444674787362369878764463410213281686695453522031111103251 Sequence: LRQSISFSGLPSGRRTSPPPPGLAGPSLSSSSFSPSSSPPPPGDLPLSPSAFSAAPGTPL Prediction: ------+--+-++++--------------------------------------------- Confidence: 013021421534344200020000000000000000000000000000000000001102 Sequence: ARRDPTPVCCPSCRRATPISVWGPLGGLVRTPSVQSLGSDPDEYASSGSSLGGSDSPVFE Prediction: -------------------------+---------------------------------- Confidence: 012132100100002001012211032212100000111002000001211100000000 Sequence: AGVFAPPQPVAAPRRLPIFNRISVSE Prediction: ------++-+------------+-+- Confidence: 00000034253033203113303132 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.