| Detail-Interactions: | |
| RAID ID: | RPI00000637 |
|---|---|
| RNA/Protein Symbol 1: | MIR200C(miR-200c) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | TUBB3 |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Immunoprecipitation, Immunohistochemistry |
| Tissue: | A2780 cells, OVCAR-3 cells, A2780-CIS cells, A2780-ADR cells |
| PMID: | 23394580 |
| Detail description: | Through theanalysis of 3`UTR-associated complexes, we found that the miR-200c can increasethe association of the RNA binding protein HuR with TUBB3 mRNA, whereas HuR binding enhanced TUBB3 mRNA translation. |
| The Predicated Binding Sites between MIR200C and TUBB3 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR200C Location | TUBB3 Location |
| The Predicated Binding Sites between MIR200C and TUBB3 | ||||
| By RIsearch | Structure | MIR200C Location | TUBB3 Location | Score |
| The Predicated Binding Sites of TUBB3 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|Q13509|TBB3_HUMAN Tubulin beta-3 chain OS=Homo sapiens GN=TUBB3 PE=1 SV=2 Sequence: MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYV Prediction: ----------+--+---------------------+---------+------+-+++++- Confidence: 929994937443452646395599698666542435544547376672823253555866 Sequence: PRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVV Prediction: -+---------+-++-++-------+------------+++-+----------------- Confidence: 369989796445446695364327759866778322227553742223786997599576 Sequence: RKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVV Prediction: +-------------------++++++--+----+-+-----------------+------ Confidence: 623643887848848333335485943567662675332672753683564236623479 Sequence: EPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSL Prediction: -----+-----------------------------+--+-++-------+---+--+++- Confidence: 863364739658985555338975889589892365436445567458848246247675 Sequence: RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMM Prediction: +---------++---------+-----------+-+++++++------------------ Confidence: 743437243775767478487596899998825649586578572645844677793576 Sequence: AACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRG Prediction: -----++-++-+-----+-+-------------++++++----------+--------+- Confidence: 746438628544762656584242598899868547755289679756957876384472 Sequence: LKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS Prediction: -+-+++-----------------------++----------------------------- Confidence: 384545354222782487328356824475528565755668688979347223624773 Sequence: EYQQYQDATAEEEGEMYEDDEEESEAQGPK Prediction: --------------------------+--+ Confidence: 433323452667555846876664256327 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q13509|TBB3_HUMAN Tubulin beta-3 chain OS=Homo sapiens GN=TUBB3 PE=1 SV=2 Sequence: MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYV Prediction: -+---+-+-++++++-++---------------------------------------++- Confidence: 656484955374456544572698565226313223775458587352446356531574 Sequence: PRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVV Prediction: ++--------------++--+-+--+----------+++++-+--++------------- Confidence: 362836483527421745343332357516788222368662711472175896897349 Sequence: RKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVV Prediction: ++---------------+-++++++++-+---------+--+-----------+------ Confidence: 364631644415728715336477973362674292733214413461355444525726 Sequence: EPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSL Prediction: ---+-------------------------------+-----------------++-+--- Confidence: 411573726137962638578953437467751254341611425228812553454216 Sequence: RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMM Prediction: +---+-+---+----------+-+-----------+--++-+------------------ Confidence: 625446433844786373234563364512512244117325231261872574342244 Sequence: AACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRG Prediction: -+---++-+--+-----------------------+++-----------+----+--+++ Confidence: 333348716153574693655622652437538144641127728335836121511785 Sequence: LKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS Prediction: +++------+-+--+----------+++++++-+-------------------------- Confidence: 375115353425444277245522453436764532354556587977678879959998 Sequence: EYQQYQDATAEEEGEMYEDDEEESEAQGPK Prediction: -----------------------------+ Confidence: 966899999999998999999989996846 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q13509|TUBB3 Sequence: MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYV Prediction: -------+-++-+++------------------------------------+--+++++- Confidence: 042002152574777234302100000300123234311100100214022634967864 Sequence: PRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVV Prediction: -+--------------+----++--+-------------++-+-+--------------- Confidence: 361010103122141152443561260132113424444653535243113000100100 Sequence: RKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVV Prediction: ----+-----+-----+----+++++---------------------------+------ Confidence: 121053443362240153244766851130001201112112001012011135002100 Sequence: EPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSL Prediction: -----------------------------------+--++++++---------+---++- Confidence: 301303010110002010001002410020003205125655560430010005302553 Sequence: RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMM Prediction: ++--+------+---------+-------------+-----+--+--+------------ Confidence: 854472422045000402321612210331342326334236015135013203202401 Sequence: AACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRG Prediction: ---+-++-++-------+++------------------------------------+-+- Confidence: 021538648522133127574423100000000203312210210033043104315283 Sequence: LKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS Prediction: -+-----------------+---------++----------------------------- Confidence: 361111212434203200350131032137531313342240010010000002000000 Sequence: EYQQYQDATAEEEGEMYEDDEEESEAQGPK Prediction: ------------------------------- Confidence: 1010000000000000000000000000120 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYV Prediction: ++++-+-+-++++++-++---------+----+-+------------+++-++---+++- Confidence: 473604292899769044000000001501023032000000001003550451123952 Sequence: PRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVV Prediction: ++--------+-----++-+-+++-+-------++++++++++++++-----------+- Confidence: 461000000050020044252744240000000569999996958751000000000160 Sequence: RKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVV Prediction: -+----+++-----------+++++++++--------+--++++-+-------------- Confidence: 360101543210010001309999989463000000040086530621101211002000 Sequence: EPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSL Prediction: --+------+---------------+-++---++++--++++-+--+-----------+- Confidence: 014100000420000000000000080490034456027635340370000001100033 Sequence: RFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMM Prediction: +++++++++------+--+++-------++-+---+-+---+-++--+---------+-- Confidence: 988696459110003600474100000065150015041004135005012010003511 Sequence: AACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRG Prediction: -++++++-+-+-----------++++++++---+-+++-+++-+---+-+--++++++++ Confidence: 045468937330020111012046554354120708950444381033030358999943 Sequence: LKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVS Prediction: ++++--+--+-------------+++++--+++++--+---------------------- Confidence: 645411302620000300000014444423795742362100000000000000000000 Sequence: EYQQYQDATAEEEGEMYEDDEEESEAQGPK Prediction: ------------------------------ Confidence: 000000000000000000000000000012 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.