| Detail-Interactions: | |
| RAID ID: | RPI00000632 |
|---|---|
| RNA/Protein Symbol 1: | MIR579(miR-579) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | TIAL1(TIAR) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Luciferase Reporter Assay, RNP Immunoprecipitation, Immunoblotting |
| Tissue: | THP-1 cells, Log-phase cells |
| PMID: | 20435889 |
| Detail description: | The process required to disrupt protein synthesis followed Toll-like receptor 4 (TLR4)-dependent induction of microRNA (miR)-221, miR-579, and miR-125b, which coupled with RNA-binding proteins TTP, AUF1, and TIAR at the 3`-untranslated region to arrest protein synthesis. TTP and AUF1 proteins linked to miR-221, whereas TIAR coupled with miR-579 and miR-125b. |
| The Predicated Binding Sites between MIR579 and TIAL1 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR579 Location | TIAL1 Location |
| The Predicated Binding Sites between MIR579 and TIAL1 | ||||
| By RIsearch | Structure | MIR579 Location | TIAL1 Location | Score |
| The Predicated Binding Sites of TIAL1 | |
| RBPBD | RRMx3 |
| RsiteDB | |
| BindN |
>sp|Q01085|TIAR_HUMAN Nucleolysin TIAR OS=Homo sapiens GN=TIAL1 PE=1 SV=1 Sequence: MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRD Prediction: ------+-++-+--+--+---------------+-++-+------+-------------- Confidence: 998754437465424627682699997994474537637443432524429898967838 Sequence: AAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPF Prediction: --------+-++---+--+--+-+++++++++++---------------+---+------ Confidence: 746765445586643447572446788999977634759999782437253466274536 Sequence: GKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAP Prediction: -+----+--+---+++++-+-------------------------++-++++-+++++++ Confidence: 847256588555375979283473755277765879586732534763958737876878 Sequence: KSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEK Prediction: ++++++++++-+-------++-++-+-------+-------+------------------ Confidence: 978888868656877784248287358264464636332576636357858879396652 Sequence: GYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVY Prediction: --+--+------------+-+-++------+----+-++--++----------------+ Confidence: 624266623332582878485455632388584365444344637328424234334265 Sequence: GNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIP Prediction: -+-+++-+-------------+-+-------------+---------+-++-+------- Confidence: 362566442563852744346645332347646423253637655537247264334763 Sequence: PPNQAGYGMASYQTQ Prediction: --+---+---++-++ Confidence: 444233633345245 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q01085|TIAR_HUMAN Nucleolysin TIAR OS=Homo sapiens GN=TIAL1 PE=1 SV=1 Sequence: MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRD Prediction: ---------+-+-++--+--------------------+--+++++++++-+-+------ Confidence: 577676451564645755694499998995797863177134676758685684865569 Sequence: AAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPF Prediction: ------------------+-++++--+---------------+--+-------------- Confidence: 889987562712444213547855234244455787858271565349578997999687 Sequence: GKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAP Prediction: ----+-+--+++++++++++-+-+------------------------+-++-+++---- Confidence: 764438725644466779694776667488887977463742564136557613453222 Sequence: KSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEK Prediction: +-++-++-++-+---------------+--+-----------------------+--+-+ Confidence: 323314327524443462322435516451577459878896887677877618823538 Sequence: GYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVY Prediction: ++-+-+------------------------+-++-+-+--++++-+---++++++----+ Confidence: 455384764488997988572743543367656425152165441313134643332233 Sequence: GNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIP Prediction: --++++-++--+------------+++++--+--+-++--+--++++++++-++------ Confidence: 213354244334321552344122434432234132331143144447457153212221 Sequence: PPNQAGYGMASYQTQ Prediction: --+++++---++--+ Confidence: 118754522253117 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q01085|TIAL1 Sequence: MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRD Prediction: -+-----+-+---+---------------------------+++++++++++-------- Confidence: 051121453524153244301100000011202422434146568688787714001122 Sequence: AAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPF Prediction: ------+---++--+---+-++++-+++-+------------+----------------- Confidence: 103111534375226433847777455545212224040213703430000000002122 Sequence: GKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAP Prediction: ++++++++++++++++++++++++--+-----------+--+-++++++++++++++++- Confidence: 566878955888899999999959426234114212126226469967978999998874 Sequence: KSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEK Prediction: ------------------------------+-------------------------++++ Confidence: 321211113100000001023133120312502320000000000110211032415669 Sequence: GYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVY Prediction: ++++++++++-+-----+--+-+++++++++++++++++--------------------- Confidence: 988879769825312115427367769967979989776422321110111001101100 Sequence: GNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIP Prediction: ------------------------------------------------------------ Confidence: 010100000000001000010001000000001102110001101000011001111001 Sequence: PPNQAGYGMASYQTQ Prediction: ------+----+--+- Confidence: 1024025414452160 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MMEDDGQPRTLYVGNLSRDVTEVLILQLFSQIGPCKSCKMITEHTSNDPYCFVEFYEHRD Prediction: +-+++----+-+-++++++----------------++-++++++++++++-+-+------ Confidence: 415472020419299959300000000000000005739999899699991917000000 Sequence: AAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPF Prediction: -----------+--++--+++++++++++++++--+++-+-++++++------------- Confidence: 000000000024105633959999696654465224470829994940000000000000 Sequence: GKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAP Prediction: ---+++++++++++++++++-+-+---------------------+--+-++++++++++ Confidence: 000585998977748999990909000000000000000013201412809999999886 Sequence: KSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSPFGQIMEIRVFPEK Prediction: ++++-+++++---+----++++++-+-+-+++-++----------------++-++++++ Confidence: 865535444432252322455645270729991640000000000000000453965689 Sequence: GYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKESPDMTKNFQQVDYSQWGQWSQVY Prediction: ++-+-+-----------------+--+++++-+++++++++++++++++++++-++++++ Confidence: 880627000000000000000037206964929999998988875644553442584656 Sequence: GNPQQYGQYMANGWQVPPYGVYGQPWNQQGFGVDQSPSAAWMGGFGAQPPQGQAPPPVIP Prediction: ++++++-++--+-------------+-+-----+-++-+-+++++++++++++------- Confidence: 554465253135000133311000042321110434424235345467555431200100 Sequence: PPNQAGYGMASYQTQ Prediction: --------+--++++ Confidence: 000010234234345 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.