| Detail-Interactions: | |
| RAID ID: | RPI00000461 |
|---|---|
| RNA/Protein Symbol 1: | MIRLET7I(let-7) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | IGF2BP3(IGF2BPs) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | PAR-CLiP, RNA Immunoprecipitation |
| Tissue: | HEK293 cells |
| PMID: | 23069990 |
| Detail description: | however, let-7 microRNAs, |?-catenin (CTNNB1) and MYC have been proposed to be involved in their regulation. In contrast to many other RNA-binding proteins, IGF2BPs are almost exclusively observed in the cytoplasm where they associate with target mRNAs in cytoplasmic ribonucleoprotein complexes (mRNPs). |
| The Predicated Binding Sites between MIRLET7I and IGF2BP3 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIRLET7I Location | IGF2BP3 Location |
| The Predicated Binding Sites between MIRLET7I and IGF2BP3 | ||||
| By RIsearch | Structure | MIRLET7I Location | IGF2BP3 Location | Score |
| The Predicated Binding Sites of IGF2BP3 | |
| RBPBD | RRMx2, KHx4 |
| RsiteDB | |
| BindN |
>sp|O00425|IF2B3_HUMAN Insulin-like growth factor 2 mRNA-binding protein 3 OS=Homo sapiens GN=IGF2BP3 PE=1 SV=2 Sequence: MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGK Prediction: -------------------------+---------+--+------------------+-+ Confidence: 742927638342553237337826757462645675364759787883487398465547 Sequence: IELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETA Prediction: -----+---------++++-++---+---------------------------++-+--- Confidence: 624255473874233787929952772744496799986999547886474254634327 Sequence: VVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSS Prediction: -----++++++-+------------------------------+-++++-++++++++++ Confidence: 582727668653826552826638638382883979887734243767929976699899 Sequence: RQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAA Prediction: ++++++++++++------+-------------+---+-++--++++++-+-++------- Confidence: 988976857746342769499874678678864333748632765568353472223245 Sequence: EKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLK Prediction: -+--+---++--++---+-------------------------------+---+--++-+ Confidence: 252745726422782245399597646944938688969398976457667636239648 Sequence: KIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNL Prediction: +-------+---------------++-+-+-+--+------------+--------+--- Confidence: 833232527825369568472333765557357454528656764386234733765637 Sequence: QAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAI Prediction: ------------------++-++++++++++--+---------+---------------- Confidence: 387995793747877654453575588557732622242522252328498989927656 Sequence: IGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFV Prediction: --++-++-+---+------+---------+-+----------+-+-+-+-+-+------- Confidence: 546545545272745252765637362356855989254434545553856267276366 Sequence: SPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKIT Prediction: ------+-----+--+----+---+--++-------++---------++----------- Confidence: 343445585748584435657632722864246338448455752325545475869392 Sequence: GHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK Prediction: ----------+---------+++++++--++++++++++ Confidence: 747397275252822973275655446337788999998 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|O00425|IF2B3_HUMAN Insulin-like growth factor 2 mRNA-binding protein 3 OS=Homo sapiens GN=IGF2BP3 PE=1 SV=2 Sequence: MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGK Prediction: --+-+--+--+------------------+-++--++++-+-+----------------+ Confidence: 425644176443954789699945827444834514867757487556768698474143 Sequence: IELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETA Prediction: ---+-+--+-++++-++++--+-+-++-----------------------+--+------ Confidence: 224423244343445463642746554463596889989998998686653244221234 Sequence: VVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSS Prediction: ------------------------------------------------------------ Confidence: 465823566978779756739638777564653643444424222123252245673543 Sequence: RQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAA Prediction: --------------------------------++----+--------+-+--+------- Confidence: 455333354264557989696786477487455522265585662256546342545578 Sequence: EKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLK Prediction: -+-------------------------------------------------+++++++-+ Confidence: 837948233655465775799789657755536679889453641256212568647437 Sequence: KIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNL Prediction: --------+-+------------------+------------------------------ Confidence: 355676384738248658786699179274245546839868986692854865663343 Sequence: QAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAI Prediction: ---+----+-+-------+------++---------+----------------------- Confidence: 231332114343314425312234143243442341412223335679675954557332 Sequence: IGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFV Prediction: +++++++-+--------+-+---------------------------------------- Confidence: 448845646263362364154416476567937987133645674876688979798467 Sequence: SPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKIT Prediction: --+------------+----+-+++++++-+-------------+--------------+ Confidence: 224362478977253324414178987852835346439273754576666797779374 Sequence: GHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK Prediction: --------------------------+--++++++++++ Confidence: 247376567528968894784444415217545788998 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>O00425|IGF2BP3 Sequence: MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGK Prediction: --+-+--+---------------------------+++---------------------- Confidence: 248351352321000000000000320100100035772030001001100200100101 Sequence: IELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETA Prediction: ---------------+++++++---++++++-----------+----------------- Confidence: 030124002014424668765834486576701010011101632111111011121210 Sequence: VVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSS Prediction: ----+--++--------------------------------------------------- Confidence: 012051355340141013332010102103110120021110010211222102312222 Sequence: RQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAA Prediction: -+----------------------+--+-+++++++++++-+---+++-++--------- Confidence: 453131001000000000000004533736889787879516334857395342312433 Sequence: EKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLK Prediction: -+----+---++--+-----------------------------++++++++++++++++ Confidence: 251022643466325200000100000000000000000100228885886889898978 Sequence: KIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNL Prediction: +--+--+-+-+-----------------------+------------------------- Confidence: 514534727350130112211201100111213462111011001100101110010001 Sequence: QAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAI Prediction: ---------------------------------------------------------+-+ Confidence: 201000000010110011012123434422211122232110100000001112443727 Sequence: IGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFV Prediction: +++++++-++-++-++--++-----------------+-+++------------------ Confidence: 879977748608736623571330000000010001354557223000100110000212 Sequence: SPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKIT Prediction: ---------------+++++++++++++++++-++-++---------------------- Confidence: 021000111030131668576788989888952973773333114122320001000013 Sequence: GHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK Prediction: -++++++----------------------++++++++++- Confidence: 4656755322220201001020111243257698989990 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MVMGLGVLLLVFVLGLGLTPPTLAQDNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLT Prediction: ------------------------+---+--++--++-+++++++-++++++++++++++ Confidence: 322020100000000000021302400060075037527666846355557557989999 Sequence: SPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRA Prediction: ++++++++-+-+----------+++++++++++++++++-++++++++++++++++++++ Confidence: 999999472434200003020257995999999976999258545999999999989994 Sequence: TAGFRNVVVACENGLPVHLDQSIFRRP536942163412838571816831616599449 Prediction: ++-+++++++-++++++--+----++-+++++++++++++++++++++++++++++++++ Confidence: 661699499824394533060100430444444444444444444444444444444444 Sequence: 847511311228165739233191893226837652942749199184466924873622 Prediction: ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 444444444444444444444444444444444444444444444444444444444444 Sequence: 82952833439485268622825299749927343553629871665798511 Prediction: +++++++++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 44444444444444444444444444444444444444444444444444444 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.