| Detail-Interactions: | |
| RAID ID: | RPI00000429 |
|---|---|
| RNA/Protein Symbol 1: | MIRLET7A1(let-7) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | IGF2BP1(IGF2BPs) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | PAR-CLiP, RNA Immunoprecipitation |
| Tissue: | HEK293 cells |
| PMID: | 23069990 |
| Detail description: | however, let-7 microRNAs, |?-catenin (CTNNB1) and MYC have been proposed to be involved in their regulation. In contrast to many other RNA-binding proteins, IGF2BPs are almost exclusively observed in the cytoplasm where they associate with target mRNAs in cytoplasmic ribonucleoprotein complexes (mRNPs). |
| The Predicated Binding Sites between MIRLET7A1 and IGF2BP1 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIRLET7A1 Location | IGF2BP1 Location |
| The Predicated Binding Sites between MIRLET7A1 and IGF2BP1 | ||||
| By RIsearch | Structure | MIRLET7A1 Location | IGF2BP1 Location | Score |
| The Predicated Binding Sites of IGF2BP1 | |
| RBPBD | RRMx2, KHx4 |
| RsiteDB | |
| BindN |
>sp|Q9NZI8|IF2B1_HUMAN Insulin-like growth factor 2 mRNA-binding protein 1 OS=Homo sapiens GN=IGF2BP1 PE=1 SV=2 Sequence: MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGK Prediction: -------------+--------------+------+--+--------------------+ Confidence: 742927737343753658528897627253624665354758787996598488424247 Sequence: VELQGKRLEIEHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETA Prediction: -+-+-++--------+++++++-+-+------+--------------------++-+--- Confidence: 652637752653332899989926572622284689976887335276563245624327 Sequence: VVNVTYSNREQTRQAIMKLNGHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQG Prediction: ----++++++++++---+-+---------+------------+++++++++++++++++- Confidence: 582667789876745455746226446685832879868624568599798699778972 Sequence: SPVAAGAPAKQQQVDIPLRLLVPTQYVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAA Prediction: +------+-+--------+-------------+---+-++--++++++-+-++------- Confidence: 725332263822263869498863528657864333748632765568353472223246 Sequence: EKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLKILAHNNFVGRLIGKEGRNLK Prediction: -+--+-+++----++--+----------+++++----------------+---+--++-+ Confidence: 356647456222265455899798633565564533859398976457667636239648 Sequence: KVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREAYENDVAAMSL Prediction: +-------+---------------++-+-+-------+---------+------------ Confidence: 833232427725338458372233766576468536569435753386364877876436 Sequence: QSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAI Prediction: ------------------+++--++-+++-+++--++----------------------- Confidence: 225885894777886636557345525564654435425625686879599979587555 Sequence: IGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFF Prediction: --++-++-+--++--+-+-+----+++-++-+----------+-+-+-+-+-+------- Confidence: 547736636275744744765324555266864989254434545553856276257465 Sequence: GPKEEVKLETHIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKII Prediction: ------+-----+---+---+---+--++------------------++----------- Confidence: 753453584349673463439532722864247459389577752325545475979499 Sequence: GHFYASQMAQRKIRDILAQVKQQHQKGQSNQAQARRK Prediction: ------+--++--+------+++++++++++++++++ Confidence: 9693925456628639782865668959789894987 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q9NZI8|IF2B1_HUMAN Insulin-like growth factor 2 mRNA-binding protein 1 OS=Homo sapiens GN=IGF2BP1 PE=1 SV=2 Sequence: MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGK Prediction: -++-+-++--+--------------------++--++++-+-+----------------- Confidence: 544554376345934999799976828521743524857658586666888798454262 Sequence: VELQGKRLEIEHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETA Prediction: ---+-+--+-+++--------+-+-++-----------------------++-++++++- Confidence: 425433143233424335253567467541593689979996777576275427777461 Sequence: VVNVTYSNREQTRQAIMKLNGHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQG Prediction: ----------------------------------------------+------------- Confidence: 153815555685587787948635478565844856657536646432434522565357 Sequence: SPVAAGAPAKQQQVDIPLRLLVPTQYVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAA Prediction: --------------------------------+++-+-+--------+-+--+------- Confidence: 666686656375577999898996479566337641535584552256466352543548 Sequence: EKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLKILAHNNFVGRLIGKEGRNLK Prediction: -+------------------------------------------++---+-+++++++-+ Confidence: 738958123734553854999999765634436967889665454433151789868537 Sequence: KVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREAYENDVAAMSL Prediction: --------+-+------------------+------------------------------ Confidence: 364655473748237669886788169264145336629759997893886742444324 Sequence: QSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAI Prediction: +-----------------+-+----++---------++---------------------- Confidence: 413443331422523412415112245224224321341246647989894742252242 Sequence: IGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFF Prediction: +++++++-+----------+--+-------------+----------------------- Confidence: 547855647153272371454243124337947977333444464947875648277245 Sequence: GPKEEVKLETHIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKII Prediction: ----------------------+++++++-+--------+----+--------------- Confidence: 411241155757285215412347754544746356328452525665665788689482 Sequence: GHFYASQMAQRKIRDILAQVKQQHQKGQSNQAQARRK Prediction: ---------------------------++++++++++ Confidence: 3651553585489598989956643244554775988 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q9NZI8|IGF2BP1 Sequence: MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGK Prediction: -++-+-------------------------------++---------------------- Confidence: 258251442321010000000000220100100034772030001000000100100101 Sequence: VELQGKRLEIEHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETA Prediction: --------------++++++++---++-+++----------------------------- Confidence: 020112001022325767855834488477702100021101210000100013011100 Sequence: VVNVTYSNREQTRQAIMKLNGHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQG Prediction: ----+---+---------------------------------------+-+----++--- Confidence: 011051235242331112111000001001000000000011124444645343355422 Sequence: SPVAAGAPAKQQQVDIPLRLLVPTQYVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAA Prediction: ---------------------------+-+++++++++++-++--+++-+--+------- Confidence: 443112112202000010100004443745789787869517544857384452312321 Sequence: EKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLKILAHNNFVGRLIGKEGRNLK Prediction: -+----++++++--+-----------------------------++++++++++++++++ Confidence: 252133666666225110000111000000100000010101218895987889898979 Sequence: KVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREAYENDVAAMSL Prediction: +-+---+-+-------------------------+------------------------- Confidence: 615414615340120112111101100111213463111012001110110100001100 Sequence: QSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAI Prediction: -----------------------+-+-+--+----------------------++-+--+ Confidence: 312011112011111122222125453532532221232121101100201225545425 Sequence: IGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFF Prediction: ++++++++++-++-++--++----------------++++++--+--------------- Confidence: 868785879516745534672210000000010024576656345121201110111221 Sequence: GPKEEVKLETHIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKII Prediction: ---------------+++++++++++++++++-++-++------+--------------- Confidence: 121111112131131678576788989888952962752323225131310000000012 Sequence: GHFYASQMAQRKIRDILAQVKQQHQKGQSNQAQARRK Prediction: +++++++-------------------+++++++++++- Confidence: 57678554212201110010122224557998889880 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MNKLYIGNLNESVTPADLEKVFAEHKISYSGQFLVKSGYAFVDCPDEHWAMKAIETFSGK Prediction: --+-------------------------+------------------------------- Confidence: 004221232122000000000000001231010003001000000101100100000103 Sequence: VELQGKRLEIEHSVPKKQRSRKIQIRNIPPQLRWEVLDSLLAQYGTVENCEQVNTESETA Prediction: ----------+-++++++++++++-++--+------------------------------ Confidence: 122101222043565666745646364233200000000000000000020100000000 Sequence: VVNVTYSNREQTRQAIMKLNGHQLENHALKVSYIPDEQIAQGPENGRRGGFGSRGQPRQG Prediction: ----+++++++-+--+-----++--+---------------+++++++++++++++++++ Confidence: 002044444332322313220330041001001010001023435897888879755746 Sequence: SPVAAGAPAKQQQVDIPLRLLVPTQYVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAA Prediction: ++---+-+----------+-----++--++++++++++++++++++++++++-++++++- Confidence: 442314343312200000500000440153648568879958553749764424656530 Sequence: EKAISVHSTPEGCSSACKMILEIMHKEAKDTKTADEVPLKILAHNNFVGRLIGKEGRNLK Prediction: -+------+--+-------------------++--+---+-----++-++++++++++++ Confidence: 040000005214231010000000000000053004011500033663375769999777 Sequence: KVEQDTETKITISSLQDLTLYNPERTITVKGAIENCCRAEQEIMKKVREAYENDVAAMSL Prediction: +-+++++++++-+-------+--------+----+----------+-------------- Confidence: 525545448452321001004230300004231232110100000412003322000100 Sequence: QSHLIPGLNLAAVGLFPASSSAVPPPPSSVTGAAPYSSFMQAPEQEMVQVFIPAQAVGAI Prediction: -------------------------------------------+--------------++ Confidence: 000001120000000320100001001000000001000000243110000000022366 Sequence: IGKKGQHIKQLSRFASASIKIAPPETPDSKVRMVIITGPPEAQFKAQGRIYGKLKEENFF Prediction: ++++++++++-+++++-+++-+-+++++---+-----++---------+---------+- Confidence: 679788687636555428463514564433051110235212100000400020201342 Sequence: GPKEEVKLETHIRVPASAAGRVIGKGGKTVNELQNLTAAEVVVPRDQTPDENDQVIVKII Prediction: ---------------++++++-+++++++++--+-++---------+--+---+------ Confidence: 312011001020103455356355999975620523400311001042230113000100 Sequence: GHFYASQMAQRKIRDILAQVKQQHQKGQSNQAQARRK Prediction: -----+++------------+--++++++++++++++ Confidence: 0102245403201210313273285688876765655 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.