| Detail-Interactions: | |
| RAID ID: | RPI00000428 |
|---|---|
| RNA/Protein Symbol 1: | MIR221(miR-221) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | HNRNPD(AUF1) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Luciferase Reporter Assay, RNP Immunoprecipitation, Immunoblotting |
| Tissue: | THP-1 cells, Log-phase cells |
| PMID: | 20435889 |
| Detail description: | The process required to disrupt protein synthesis followed Toll-like receptor 4 (TLR4)-dependent induction of microRNA (miR)-221, miR-579, and miR-125b, which coupled with RNA-binding proteins TTP, AUF1, and TIAR at the 3`-untranslated region to arrest protein synthesis. TTP and AUF1 proteins linked to miR-221, whereas TIAR coupled with miR-579 and miR-125b. |
| The Predicated Binding Sites between MIR221 and HNRNPD | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR221 Location | HNRNPD Location |
| The Predicated Binding Sites between MIR221 and HNRNPD | ||||
| By RIsearch | Structure | MIR221 Location | HNRNPD Location | Score |
| The Predicated Binding Sites of HNRNPD | |
| RBPBD | RRMx2 |
| RsiteDB | |
| BindN |
>sp|Q14103|HNRPD_HUMAN Heterogeneous nuclear ribonucleoprotein D0 OS=Homo sapiens GN=HNRNPD PE=1 SV=1 Sequence: MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGS Prediction: ---------------+-----+--------------------+++++++++++++++-++ Confidence: 847756665653454643432723334674765224433332865697879685687278 Sequence: AESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKF Prediction: -+++--+-+-++-------+++++++++-++-++-------------+-++--+------ Confidence: 378633936268232123158887867636736734339887672115176246546439 Sequence: GEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEP Prediction: -------------+-+++----------------------+-+------++-++-+++-- Confidence: 979978482742363969435598936342752646424275541452359296185822 Sequence: VKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIM Prediction: -+----------------+-----------------+++++++----------------- Confidence: 451967555212112217575787574943847345588899952449485964472153 Sequence: EKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQ Prediction: -----+----+---+---++-++++-+++-++-++-++-+++++++++++++-+++---- Confidence: 311336436174476654581576525662693682692969887888557637661111 Sequence: GYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY Prediction: -+-++-++++-+-++--+-++-+++++-+--++++++++++-+++++++++++-+ Confidence: 3636627656271582163563656654732677688597927996699877725 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q14103|HNRPD_HUMAN Heterogeneous nuclear ribonucleoprotein D0 OS=Homo sapiens GN=HNRNPD PE=1 SV=1 Sequence: MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGS Prediction: ------------------------------------------------------------ Confidence: 527879887254687898999899989797987876674432134537574656976755 Sequence: AESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKF Prediction: ----------++------+-+++++------------+---++----------------- Confidence: 925586262435342534334333512256572345237153486259547995899558 Sequence: GEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEP Prediction: ------+--+++++++++++-+-+-----------------------+-++++------- Confidence: 786627622456475778586674746568877856326224221245387374532244 Sequence: VKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIM Prediction: --+-+-++-----------------------+---++++++++++-+-+----------- Confidence: 735544466513946579588778664762732314557788968568466868887687 Sequence: EKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQ Prediction: --------------+-+-++-++++++++-+++-+++++++++++++++++--+++++-+ Confidence: 944634566223156341562476768761444154686757746657654123344414 Sequence: GYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY Prediction: -+-++-++-+++--+-+++++++++++----++++++++++++++++++++++++ Confidence: 1314424524342151464454453332122464543477768989788888758 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q14103|HNRNPD Sequence: MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGS Prediction: ------+----------------------------------------------------- Confidence: 102232533411000000000000000000000000000000000000000000000000 Sequence: AESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKF Prediction: -------------------------------------+---++-++-------------- Confidence: 000000000000000000000000110000201110151425726630210100141131 Sequence: GEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEP Prediction: ---------+++++++++++++---------------+-++-+++++++++++++++++- Confidence: 120001114688999999987943333000001221154574699778899988985663 Sequence: VKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIM Prediction: --+-+-++----------------------------+++++++++-+-+----------- Confidence: 348374861420000000102101000000000103757789987483623111000100 Sequence: EKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQ Prediction: --+++++++++++-+++--+++++++++++++++++++++++++++++++++--+----- Confidence: 047697889985949764486657678899999999999999999977766544521331 Sequence: GYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY Prediction: -------------------------------------++++++++++++++++++- Confidence: 11221211242231210210111102100000333347566788999999999880 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MSEEQFGGDGAAAAATAAVGGSAGEQEGAMVAATQGAAAAAGSGAGTGGGTASGGTEGGS Prediction: --------+++----------+-------------------------------------- Confidence: 000113034653110000022300000000000010000000000031000000000223 Sequence: AESEGAKIDASKNEEDEGHSNSSPRHSEAATAQREEWKMFIGGLSWDTTKKDLKDYFSKF Prediction: ------+-------+---+-++++++-+--+++--+-+++-+++-+-------------- Confidence: 000033312001224213335654843522474325183939993900000000000000 Sequence: GEVVDCTLKLDPITGRSRGFGFVLFKESESVDKVMDQKEHKLNGKVIDPKRAKAMKTKEP Prediction: ----+-++++++++++++++-+++---------------++++++++++++++++++++- Confidence: 000141889999959999993959000000010001022794579479999997786652 Sequence: VKKIFVGGLSPDTPEEKIREYFGGFGEVESIELPMDNKTNKRRGFCFITFKEEEPVKKIM Prediction: ++++++++++++----------------++-++++++++++++++-+-+----------- Confidence: 568497999396000000000000000038399787895999999290800000000000 Sequence: EKKYHNVGLSKCEIKVAMSKEQYQQQQQWGSRGGFAGRARGRGGGPSQNWNQGYSNYWNQ Prediction: -----++--+-++-++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 002035302615909799998999999799999999898999799999894557554434 Sequence: GYGNYGYNSQGYGGYGGYDYTGYNNYYGYGDYSNQQSGYGKVSRRGGHQNSYKPY Prediction: +++++++++-+++++++++++-+++-+++++++++++++++++++++++++++++ Confidence: 4645644553453455454353444344648569999999999999999999784 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.