| Detail-Interactions: | |
| RAID ID: | RPI00000427 |
|---|---|
| RNA/Protein Symbol 1: | MIR18A(miR-18a) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | HNRNPA1(hnRNP A1) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Co-RNP Immunoprecipitation, Cross-linking assay |
| Tissue: | |
| PMID: | 17558416 |
| Detail description: | Here we show that hnRNP A1 binds specifically to the primary RNA sequence pri-miR-18a before Drosha processing. |
| The Predicated Binding Sites between MIR18A and HNRNPA1 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR18A Location | HNRNPA1 Location |
| The Predicated Binding Sites between MIR18A and HNRNPA1 | ||||
| By RIsearch | Structure | MIR18A Location | HNRNPA1 Location | Score |
| The Predicated Binding Sites of HNRNPA1 | |
| RBPBD | RRMx2 |
| RsiteDB | |
| BindN |
>sp|P09651|ROA1_HUMAN Heterogeneous nuclear ribonucleoprotein A1 OS=Homo sapiens GN=HNRNPA1 PE=1 SV=5 Sequence: MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFV Prediction: -+++++++-++--+--------------+-+-+-------------+-+++++++-+--- Confidence: 568776773542762977667344223254725742493758799852687998944257 Sequence: TYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHH Prediction: --------------+-++---+----++--+++++++-+-+-----------+------- Confidence: 429598897764249348424555255723797677725366282297645753434444 Sequence: LRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKA Prediction: -------------------+++++++--------------+-------+-+--+---++- Confidence: 828477347297885862587979894255957987738647794333446525327863 Sequence: LSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGS Prediction: -++----+-+++++++++++++++-+-+++--+----+-++++-+-++++++++++++++ Confidence: 457232362899999997958665292666224322482585629367568978779568 Sequence: GDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGG Prediction: +--++---+---+-++++++++++++++++++++++++++++++++++++++-+++++++ Confidence: 522654345332825667599889969796576957758697796885665525545556 Sequence: GSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGS Prediction: +++++++++-+++----+++++++--+-+--+++++++++++++-+++-+-+++++++++ Confidence: 585874576276422325668656225473276759796786653574372877579789 Sequence: SSSSSYGSGRRF Prediction: +++++++++++- Confidence: 999999984874 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P09651|ROA1_HUMAN Heterogeneous nuclear ribonucleoprotein A1 OS=Homo sapiens GN=HNRNPA1 PE=1 SV=5 Sequence: MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFV Prediction: -+++----------+-+-++-----------------------+--+++++++++++-+- Confidence: 277423537455624735347626966799689946877672743475767787858567 Sequence: TYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHH Prediction: +-------------+------+--+-++++++--+----------+-+-++--------- Confidence: 556557887875643441334325627864552132264446484565356641495769 Sequence: LRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKA Prediction: --------------+--+++++++++++-+-+-----------------------+-+++ Confidence: 948996646556284343354666795855846776888979895563553522455755 Sequence: LSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGS Prediction: -++--++++++++++++++++++++++++--------+--+-+++-----++-------+ Confidence: 145214353677974867647543574552121112152142364121224423111225 Sequence: GDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGG Prediction: --++++++++-++--+-+-++--+++++-+++-++++-++++++++++++++++-+++++ Confidence: 224444333423521413155214634413442544516656343563454433157755 Sequence: GSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGS Prediction: ++++++++-++-++-++++++-++---+++++++-+-+--+++--++--+-+++++++++ Confidence: 555654442451633436757146112354664425131234511351632365647565 Sequence: SSSSSYGSGRRF Prediction: ++++++++++++ Confidence: 454456685785 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P09651|HNRNPA1 Sequence: MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFV Prediction: --+-----------+-+-++-++---------+-------------++++++++++++++ Confidence: 026223423214137264684673121030125223123001121378999998999897 Sequence: TYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHH Prediction: +--+----------+-++++++++++++++++++++++++++++++-+-++--------- Confidence: 742501000231115467579967969979798656686686666737476243000000 Sequence: LRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKA Prediction: ------------------++++++++++-+-+-------------+++++++++++++++ Confidence: 000211211100000001585889998738462311100000003868678998695985 Sequence: LSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGS Prediction: ++++++-+++++++++++++++++++++-++++++++++++-+++++++++++++++--- Confidence: 579656455877788899999889886745667886775664658666777855555322 Sequence: GDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGG Prediction: -----------++--+--+----++++-+++-++++++++++++++++++++++++++++ Confidence: 122223233346634544544435565475647887888868887576576567587686 Sequence: GSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGS Prediction: ++++++--------------+---+-----+----++---++-+-----------+++++ Confidence: 876565322210210122225344533434534346744455253221031134255675 Sequence: SSSSSYGSGRRF Prediction: ++++++++++++- Confidence: 5677999999970 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFV Prediction: +++++-+++++++-+-+-+++-+-----------------++-+++++++-++++++-++ Confidence: 477543697683539393999292000000000000000047386998992799999294 Sequence: TYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHH Prediction: +----------------+--++--+-+++++++++++--+++++++++++++-++----- Confidence: 900000000000000035014410618797497443423467445939499939800000 Sequence: LRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKA Prediction: ------------++++++++++++++++-+-+--------------------+--+-+++ Confidence: 000000000003849978778599999919080000000000000002200360190999 Sequence: LSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGS Prediction: ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 999579789999999999999999999999999989799788999899999987884463 Sequence: GDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGG Prediction: +++++++-++++++++++++++++++++++++++++++++++++++++++++++++++++ Confidence: 584435725667676657765765856999467657565777565464567799999994 Sequence: GSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGS Prediction: +++++--------+---+------+------------------++++--+++++++++++ Confidence: 764433112223241234122232423223202322222333263642044869978797 Sequence: SSSSSYGSGRRF Prediction: ++++++++++++ Confidence: 676547555655 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.