| Detail-Interactions: | |
| RAID ID: | RPI00000416 |
|---|---|
| RNA/Protein Symbol 1: | MIR200B(miR-200b) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | ELAVL1(HuR) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Immunoprecipitation |
| Tissue: | |
| PMID: | 23223443 |
| Detail description: | Immunoprecipitation of RNA-protein complexes and luciferase reporter assays indicate that HuR antagonizes the suppressive activity of miR-200b, down-regulates miR-200b expression, and promotes VEGF-A expression. |
| The Predicated Binding Sites between MIR200B and ELAVL1 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR200B Location | ELAVL1 Location |
| The Predicated Binding Sites between MIR200B and ELAVL1 | ||||
| By RIsearch | Structure | MIR200B Location | ELAVL1 Location | Score |
| The Predicated Binding Sites of ELAVL1 | |
| RBPBD | RRMx3 |
| RsiteDB | |
| BindN |
>sp|Q15717|ELAV1_HUMAN ELAV-like protein 1 OS=Homo sapiens GN=ELAVL1 PE=1 SV=2 Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHS Prediction: -------------+----+-----------------+------------+--+------- Confidence: 621636666857774123932668458523622547646423772731465667365423 Sequence: LGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQK Prediction: --+--------+---+-----+--+-++++-+--+-+--+--------------++---- Confidence: 455668439266363666324543837877483163721638618939381352873112 Sequence: DVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPIT Prediction: ----------+-----------+--++-----+--++------------++++++++--- Confidence: 895796427874734196575253368656476545625523772231368675875142 Sequence: VKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNA Prediction: -----++++-+----------++-++----------+-+--------------------- Confidence: 816646557262567743932655883321722142777533878856356235251213 Sequence: SSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAM Prediction: ++----------------------------------+---+++-+--------------- Confidence: 766469996698378779997999987867246288555155838546592535476787 Sequence: AIASLNGYRLGDKILQVSFKTNKSHK Prediction: ---+-+-++----------+++++++ Confidence: 87655655755337838257689787 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q15717|ELAV1_HUMAN ELAV-like protein 1 OS=Homo sapiens GN=ELAVL1 PE=1 SV=2 Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHS Prediction: --+-----------------+-+-++--+--------------------+--++++++++ Confidence: 325121412536424344234546557746954789589947978751771455746465 Sequence: LGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQK Prediction: +++-+-+--------------------+++-+-++++---------+-+-++-------- Confidence: 648657566467888588557284253363362864443446557645647754139567 Sequence: DVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPIT Prediction: ---------------+----+++++++++-+-+--------------------------+ Confidence: 997899555755718545223365568484676676488887995352643353235435 Sequence: VKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNA Prediction: -++--++++++-------------+-------++-+------------------------ Confidence: 564224344442456743413225422332213323241465547645566555385457 Sequence: SSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAM Prediction: --------++---------------------+-+--+++++++++++-+-+--------- Confidence: 578769384477279668897999787784547624634556637486686555379999 Sequence: AIASLNGYRLGDKILQVSFKTNKSHK Prediction: ---------------+-++++++-++ Confidence: 98757274345224665756638258 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q15717|ELAVL1 Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHS Prediction: ------------------+-+---++-+++-----------------+++--++++++++ Confidence: 024000120122301114507341853675010000100201033235572456878989 Sequence: LGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQK Prediction: +++++-+--+--+--+--++-++++-++++++++++++++++----+-+-++--+----- Confidence: 999874733621523632864756746877676998998885442172727634630100 Sequence: DVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPIT Prediction: ----------+--+++-+-++-++++++++++++-++-------------------+--+ Confidence: 000001232454475815365498789878879538614021124104112334236315 Sequence: VKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNA Prediction: -++++++++----------------------+---++----------------------- Confidence: 499668767443330111011131433214054116734211110121120100023131 Sequence: SSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAM Prediction: -------------------------+-++-+--+---++++++++++++++++++-+--- Confidence: 221120312401300000001101364654543811255678889887879579516211 Sequence: AIASLNGYRLGDKILQVSFKTNKSHK Prediction: --+--+-++-++++++++++++++++- Confidence: 225236377478966979889988980 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHS Prediction: +++++-++--+++++++++-+++-+++++-----------------++++++++++-+++ Confidence: 468942653146797793939791999393000000000000000069499999993899 Sequence: LGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQK Prediction: +++-+-+----------------++---+-++-++++++++++---+-+-+++-++---- Confidence: 999191800000000000000004310353492999999988732341608992840000 Sequence: DVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPIT Prediction: ------------+++++++++++++++++-+-+---------------------+--+-+ Confidence: 000000000000584966766758899890909000000000000000000021522637 Sequence: VKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNA Prediction: -++++++++++++++++++-++++++++++++++++++++++++++++++++++++-++- Confidence: 399899965564455535434454553466556535553565666676556654842432 Sequence: SSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAM Prediction: ------+-+++-+-----------------++++++++++-++++++-+-+--------- Confidence: 312120507570310000000000000000474989864506499990918000000000 Sequence: AIASLNGYRLGDKILQVSFKTNKSHK Prediction: -------++--++-++-+++++++++ Confidence: 00000005730793592999999789 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.