| Detail-Interactions: | |
| RAID ID: | RPI00000414 |
|---|---|
| RNA/Protein Symbol 1: | MIR23A(mir-23a) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | CREB5(CREB) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Luciferase Reporter Assay, qPCR, Western Blot |
| Tissue: | glioma cell line(U87MG, T98G, A172, and U251), cervical carcinoma cell line HeLa |
| PMID: | 23019365 |
| Detail description: | Moreover, mir-23a was confirmed as a functional downstream target of CREB in glioma cell growth and cell survival. |
| The Predicated Binding Sites between MIR23A and CREB5 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR23A Location | CREB5 Location |
| The Predicated Binding Sites between MIR23A and CREB5 | ||||
| By RIsearch | Structure | MIR23A Location | CREB5 Location | Score |
| The Predicated Binding Sites of CREB5 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|Q02930|CREB5_HUMAN Cyclic AMP-responsive element-binding protein 5 OS=Homo sapiens GN=CREB5 PE=1 SV=3 Sequence: MIYEESKMNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLKFPSIKTDNMLSDQTP Prediction: ------+------+------++++++------------------+--+-+--+-----++ Confidence: 896773442333855665254446673437868684242334284526562244434264 Sequence: TPTRFLKNCEEVGLFSELDCSLEHEFRKAQEEESSKRNISMHNAVGGAMTGPGTHQLSSA Prediction: ++++--+-------------------++--++++++++-----------+++-+++-++- Confidence: 756847549868888579884973566452457567864254487445275527562663 Sequence: RLPNHDTNVVIQQAMPSPQSSSVITQAPSTNRQIGPVPGSLSSLLHLHNRQRQPMPASMP Prediction: +-++------------++++++--++-++++++--+---+-------+++++++---+-- Confidence: 837544239895575364766655672677785544724562268235475855225543 Sequence: GTLPNPTMPGSSAVLMPMERQMSVNSSIMGMQGPNLSNPCASPQVQPMHSEAKMRLKAAL Prediction: -+--+-+-+-++-------+----++--------+-++---+-+-+---++-+-+-+--- Confidence: 275252634255665457854526442653425345553346346525254465676366 Sequence: THHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPHHPYPHQHQHPAHH Prediction: -------++-+------------++++++---+---+--+---------+---------- Confidence: 255364456264225868977545876872325322532522253322363223223522 Sequence: PHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQMQPTQTIQPPQ Prediction: -+-+-+++++--++-----------+--++--++--+-+--+-++-+++-+--++-+--+ Confidence: 352525555422453223273623452265225542535255544455535225445226 Sequence: PTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNE Prediction: ++++++++--------++++++++-+-++++++++++-+-----++-------------- Confidence: 699797875534422366776445482568979778645643745555262332437458 Sequence: VSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTIT Prediction: -----------------------+---+-++-+-+--+++--+--+-----------+-+ Confidence: 835834687394486935256475432526534352357522625433243267464546 Sequence: TSSSVSEVVGSSTLSQLTTHRTDLNPIL Prediction: +++-------+-+-++-+++++------ Confidence: 5752722555627477355575372599 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q02930|CREB5_HUMAN Cyclic AMP-responsive element-binding protein 5 OS=Homo sapiens GN=CREB5 PE=1 SV=3 Sequence: MIYEESKMNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLKFPSIKTDNMLSDQTP Prediction: ------+-------------++++++---------++++++-+-+----+--------+- Confidence: 463461441343714653143434573245612344865742574631252415685452 Sequence: TPTRFLKNCEEVGLFSELDCSLEHEFRKAQEEESSKRNISMHNAVGGAMTGPGTHQLSSA Prediction: +-++--+---------------------------++------------------+----- Confidence: 627816529757899789898982992486874336113444667637125332523423 Sequence: RLPNHDTNVVIQQAMPSPQSSSVITQAPSTNRQIGPVPGSLSSLLHLHNRQRQPMPASMP Prediction: +--+--------------+---------+++++---------------+-++-------- Confidence: 442312248264133211342667436243444342532233268562323422326234 Sequence: GTLPNPTMPGSSAVLMPMERQMSVNSSIMGMQGPNLSNPCASPQVQPMHSEAKMRLKAAL Prediction: ----+--------------+--------------+--+----------+----------- Confidence: 625234212212313243244245232646635353233333115133422323275797 Sequence: THHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPHHPYPHQHQHPAHH Prediction: --------------+-------------------+++-++-------------------- Confidence: 565446532115224312234245326426313234414513444524314122234345 Sequence: PHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQMQPTQTIQPPQ Prediction: -----+-------------------+---------+--++-------------------- Confidence: 125244332244112124252532233432222144116431221321333461262231 Sequence: PTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNE Prediction: +-++++++--------++-+++-+++-++++++++++----------------------- Confidence: 313364434556744354157328782446869698451678975999895664959579 Sequence: VSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTIT Prediction: ------------------------------------------------+----------- Confidence: 977958799495897974568662643674573684896648868452323358352262 Sequence: TSSSVSEVVGSSTLSQLTTHRTDLNPIL Prediction: --+------------------------- Confidence: 4235866757722623535223781365 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q02930|CREB5 Sequence: MIYEESKMNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLKFPSIKTDNMLSDQTP Prediction: ------------------+--+---+---------------------------------- Confidence: 000000000001021102513643281122130212323030301141030000000000 Sequence: TPTRFLKNCEEVGLFSELDCSLEHEFRKAQEEESSKRNISMHNAVGGAMTGPGTHQLSSA Prediction: ------------------------------------------------------------ Confidence: 000100000000000200000000000000000000000000000000000001000000 Sequence: RLPNHDTNVVIQQAMPSPQSSSVITQAPSTNRQIGPVPGSLSSLLHLHNRQRQPMPASMP Prediction: ------------------------------------------------------------ Confidence: 202000000000000000000000000000000000000000000000000000000000 Sequence: GTLPNPTMPGSSAVLMPMERQMSVNSSIMGMQGPNLSNPCASPQVQPMHSEAKMRLKAAL Prediction: ------------------------------------------------------------ Confidence: 000000110000010000010000000000000000000000000000000000000000 Sequence: THHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPHHPYPHQHQHPAHH Prediction: ------------------------------------------------------------ Confidence: 000000000100010010000000000000000000000000000000000000010000 Sequence: PHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQMQPTQTIQPPQ Prediction: ------------------------------------------------------------ Confidence: 000001012012231121000131222211010001101231010001000000000000 Sequence: PTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNE Prediction: -----+++----+-+-+++++++++++++++++++++-+--+------------------ Confidence: 012446754331546478889989999999999899648315200100000002010100 Sequence: VSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTIT Prediction: ----------------++--++-------------------------------------- Confidence: 012020100401111065345513411121100000000000000000000000000000 Sequence: TSSSVSEVVGSSTLSQLTTHRTDLNPIL Prediction: ----------------------------- Confidence: 00110111010100110021000002000 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MIYEESKMNLEQERPFVCSAPGCSQRFPTEDHLMIHRHKHEMTLKFPSIKTDNMLSDQTP Prediction: --------------------+++++-----------------+-+++++++--------- Confidence: 212101200301030000303655630101231101301212324444554300000001 Sequence: TPTRFLKNCEEVGLFSELDCSLEHEFRKAQEEESSKRNISMHNAVGGAMTGPGTHQLSSA Prediction: --++-----------------------+----+++-++++++------------------ Confidence: 235510200000000000010001202413214342544433221000021113020110 Sequence: RLPNHDTNVVIQQAMPSPQSSSVITQAPSTNRQIGPVPGSLSSLLHLHNRQRQPMPASMP Prediction: +------------------------------+-------------+-------------- Confidence: 310020000000000000000000000120243111022222121332331220232221 Sequence: GTLPNPTMPGSSAVLMPMERQMSVNSSIMGMQGPNLSNPCASPQVQPMHSEAKMRLKAAL Prediction: ------------------------++-------+----------------------+--- Confidence: 010001000000000001010100431211222410331000000100221132114012 Sequence: THHPAAMSNGNMNTMGHMMEMMGSRQDQTPHHHMHSHPHQHQTLPPHHPYPHQHQHPAHH Prediction: +-----+-+++--------------------++--------------------------- Confidence: 301122425431200000011013202131133301213101000333312000212233 Sequence: PHPQPHHQQNHPHHHSHSHLHAHPAHHQTSPHPPLHTGNQAQVSPATQQMQPTQTIQPPQ Prediction: ------++-+++++++-++++-+-++------------------------------++++ Confidence: 121323433443454423454233333210010000000000001000002212013479 Sequence: PTGGRRRRVVDEDPDERRRKFLERNRAAATRCRQKRKVWVMSLEKKAEELTQTNMQLQNE Prediction: ++++++++++++----+++++++++++++++++++++-++-+------------------ Confidence: 999999985765332056555458797697999569539524300000020111200220 Sequence: VSMLKNEVAQLKQLLLTHKDCPITAMQKESQGYLSPESSPPASPVPACSQQQVIQHNTIT Prediction: ------------------+--+------+------------------------------- Confidence: 000000000000000013533412312240001001000100000000000000001200 Sequence: TSSSVSEVVGSSTLSQLTTHRTDLNPIL Prediction: -+-------------------------- Confidence: 2312000000001000000012101101 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.