| Detail-Interactions: | |
| RAID ID: | RPI00000413 |
|---|---|
| RNA/Protein Symbol 1: | MIR23A(mir-23a) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | CREB3(CREB) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Luciferase Reporter Assay, qPCR, Western Blot |
| Tissue: | glioma cell line(U87MG, T98G, A172, and U251), cervical carcinoma cell line HeLa |
| PMID: | 23019365 |
| Detail description: | Moreover, mir-23a was confirmed as a functional downstream target of CREB in glioma cell growth and cell survival. |
| The Predicated Binding Sites between MIR23A and CREB3 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR23A Location | CREB3 Location |
| The Predicated Binding Sites between MIR23A and CREB3 | ||||
| By RIsearch | Structure | MIR23A Location | CREB3 Location | Score |
| The Predicated Binding Sites of CREB3 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|O43889|CREB3_HUMAN Cyclic AMP-responsive element-binding protein 3 OS=Homo sapiens GN=CREB3 PE=1 SV=1 Sequence: MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSP Prediction: ------------------------+------+--------------------------+- Confidence: 989896766788999999846353753335678599699793374368999999858843 Sequence: PASLNILSSSNPCLVHHDHTYSLPRETVSMDLGECEISLTGRTGFMGLAIHTFPFAESES Prediction: --+----++-----------+---+--------------+-++---------------++ Confidence: 455525655233469567224232732737688694825448444458895686673267 Sequence: CRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRR Prediction: -++++++---------------+------------------------++---+--++-++ Confidence: 297557752346977998877869882556225754898898747254626355646489 Sequence: KIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAM Prediction: ++++++++++++++++-+-----++--++-------------------------+----- Confidence: 959889889999999847422646856752722446344956879869477648628268 Sequence: VIEISNKTSSSSTCILVLLVSFCLLLVPAMYSSDTRGSLPAEHGVLSRQLRALPSEDPYQ Prediction: ------+++++++-----------------+-+-++-+--------++--+---+----- Confidence: 896832688887568999995999999787526268354475356445278343523424 Sequence: LELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEP Prediction: -------++--+-+-----------------++----------+---------------- Confidence: 957466254527242242777667674523255578558527255334877998898456 Sequence: LCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG Prediction: --+--------+-+++--+--+-+-------+-+- Confidence: 76775763835435873273264522557465243 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|O43889|CREB3_HUMAN Cyclic AMP-responsive element-binding protein 3 OS=Homo sapiens GN=CREB3 PE=1 SV=1 Sequence: MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSP Prediction: ------------------------------------------------------------ Confidence: 768898738869799999875674597888638488589586446687999999799945 Sequence: PASLNILSSSNPCLVHHDHTYSLPRETVSMDLGECEISLTGRTGFMGLAIHTFPFAESES Prediction: ------------------+----------------------------------------+ Confidence: 686858986657786355442363462225555784356577888598783444464613 Sequence: CRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRR Prediction: -+-------+---------------------+-----------------------++-++ Confidence: 435333232422554655447849987977234523686685413262257658566459 Sequence: KIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAM Prediction: ++++++++++++++++-------------------------------------------- Confidence: 839995758669498482897975799586875778257988968848796769229497 Sequence: VIEISNKTSSSSTCILVLLVSFCLLLVPAMYSSDTRGSLPAEHGVLSRQLRALPSEDPYQ Prediction: ------+++-+------------------------------------+------------ Confidence: 973422465131267879998999998538333563675578777744372675376978 Sequence: LELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEP Prediction: ------------------------------------------------------------ Confidence: 979676554643725776988575676737579999768567637766956798799798 Sequence: LCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG Prediction: ---------------+---------------++++ Confidence: 77574557828175136324213121569366565 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>O43889|CREB3 Sequence: MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSP Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000000000000000000000000 Sequence: PASLNILSSSNPCLVHHDHTYSLPRETVSMDLGECEISLTGRTGFMGLAIHTFPFAESES Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000000000000000000000000 Sequence: CRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRR Prediction: ---------------------------------------------+------+--++--+ Confidence: 000000000000000000000000002110220111210104144513410161266349 Sequence: KIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAM Prediction: +-++++++++++++++-+----------------------------+-----+--+---- Confidence: 849998999999899725102100100000023021422102121451422151462213 Sequence: VIEISNKTSSSSTCILVLLVSFCLLLVPAMYSSDTRGSLPAEHGVLSRQLRALPSEDPYQ Prediction: --+++++++++++++--------------------------------------------- Confidence: 115679999999985331000000000100100000000000000010000000000000 Sequence: LELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEP Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000000000000000000000000000000001 Sequence: LCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG Prediction: ---------------------+++-+---+-++++- Confidence: 210110000001000000301565453127455570 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSP Prediction: ------------------------------------------------------------ Confidence: 121110001000000000100011100010000000000000000000000000000000 Sequence: PASLNILSSSNPCLVHHDHTYSLPRETVSMDLGECEISLTGRTGFMGLAIHTFPFAESES Prediction: --------+---------------+----------------+------------------ Confidence: 000000013111000000111012301000221101030003222010002010000312 Sequence: CRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRR Prediction: ++++--------------------------------------------+--++-++++++ Confidence: 434332302102200000000000000000000000000000000101300342459579 Sequence: KIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAM Prediction: ++++++++++++++++++++++-++----------------------------------- Confidence: 979999999868665534444404500200020010012001000000000000210200 Sequence: VIEISNKTSSSSTCILVLLVSFCLLLVPAMYSSDTRGSLPAEHGVLSRQLRALPSEDPYQ Prediction: -----++++++++--------------------+-+-----+-+---+--+--------- Confidence: 002124465664510000011000000000001335221233152013203001000001 Sequence: LELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEP Prediction: ------------------------------------------------------------ Confidence: 000000100001012100002010000010212210100000000000000000000000 Sequence: LCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG Prediction: --------------++----------------+-+ Confidence: 00000000000200541032231000000012424 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.