| Detail-Interactions: | |
| RAID ID: | RPI00000412 |
|---|---|
| RNA/Protein Symbol 1: | MIR23A(mir-23a) |
| RNA/Protein Category 1: | miRNA |
| RNA/Protein Symbol 2: | CREB1(CREB) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Luciferase Reporter Assay, qPCR, Western Blot |
| Tissue: | glioma cell line(U87MG, T98G, A172, and U251), cervical carcinoma cell line HeLa |
| PMID: | 23019365 |
| Detail description: | Moreover, mir-23a was confirmed as a functional downstream target of CREB in glioma cell growth and cell survival. |
| The Predicated Binding Sites between MIR23A and CREB1 | |||||
| By miRanda | Structure | Match Score | Energy Score | MIR23A Location | CREB1 Location |
| The Predicated Binding Sites between MIR23A and CREB1 | ||||
| By RIsearch | Structure | MIR23A Location | CREB1 Location | Score |
| The Predicated Binding Sites of CREB1 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P16220|CREB1_HUMAN Cyclic AMP-responsive element-binding protein 1 OS=Homo sapiens GN=CREB1 PE=1 SV=2 Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPN Prediction: --------++++-+---+-------+--------------------+++++--------+ Confidence: 747522325565544665264332446273437727838355442567845272794635 Sequence: GQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTD Prediction: -++-+-------------++-+-++-+++-+--++--+--------------------++ Confidence: 556558478946635257452656557652726674542228225842352352662474 Sequence: SQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSG Prediction: +++++++-++++++++---------+---+----++--+++----++----+--+++++- Confidence: 777787526797778577267323552638522355224554235455283525455664 Sequence: QYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQV Prediction: ++------------+--+-------+----+---+++-++--+---++------------ Confidence: 567674364662544346344256257262454376536557426255252366754537 Sequence: VVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAAREC Prediction: -----+-+-++++-++-++++--------+++---+---++--+++--+--+-+--++++ Confidence: 673746263665759726555563777564553325247552395723834437245976 Sequence: RRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD Prediction: +++++++-+-------------+---------------+-- Confidence: 99989665558754985544334477689398267262523 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P16220|CREB1_HUMAN Cyclic AMP-responsive element-binding protein 1 OS=Homo sapiens GN=CREB1 PE=1 SV=2 Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPN Prediction: -+--------+---------------------------------+--------------- Confidence: 236333752334447784766462739576769958759665334631421764883831 Sequence: GQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTD Prediction: -++----------------+-+----+---+----------------------------- Confidence: 334349364734445237132432364214335247476759859997988898999963 Sequence: SQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSG Prediction: -++++-+-++++++++--+-----------------------------------+--+-- Confidence: 235572416785767647379438834641657835654456157588496333333416 Sequence: QYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQV Prediction: --------------+--+---+-------------------------------------- Confidence: 889886573582733334125335348153245754466278679344332475521225 Sequence: VVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAAREC Prediction: --------------------------------------------++-+++-+++-+++++ Confidence: 474532345132382483443984895664216338458665417714962888245656 Sequence: RRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD Prediction: +++++------------------------------------ Confidence: 96885517578735997976635687689479399493323 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P16220|CREB1 Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPN Prediction: ------------------------------------------------------------ Confidence: 010000000010000000000000000000000000000000010001110100000000 Sequence: GQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTD Prediction: ------------------------------------------------------------ Confidence: 000000000000000100100100101010100000000000000000000000000000 Sequence: SQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSG Prediction: ---++---+++---++-------------------------------------------- Confidence: 002561447772435500420000010000000000000000200000000011011100 Sequence: QYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQV Prediction: ------------------------------------------------------------ Confidence: 000000000000000000000000000000211011000000000000000000000000 Sequence: VVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAAREC Prediction: ------------------------------+---+---------++--++-+++-+++++ Confidence: 010101111212201000011001010003523451331113136624973888466667 Sequence: RRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD Prediction: ++++-------------------------------------- Confidence: 878844211200130000013213142400200001033340 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPN Prediction: -------------------------------------------+++++------------ Confidence: 223221002021333122001121022101001000000022244454233210000032 Sequence: GQTVQVHGVIQAAQPSVIQSPQVQTVQSSCKDLKRLFSGTQISTIAESEDSQESVDSVTD Prediction: --------------------------+--------------------------------- Confidence: 310000211001010000000100013232221221221121100000000001022222 Sequence: SQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSG Prediction: -+++++++-+++++++-------------+-+-+----++------------------+- Confidence: 248564761688858632331223223125241332133320100000000000210030 Sequence: QYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQV Prediction: +--------------+++++---------------------------------------- Confidence: 433211010001013535431220200000010023313100100010000100000011 Sequence: VVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAAREC Prediction: ---------------------------------------------++-++++++++++++ Confidence: 002011201200103000200000000000201200100122023640953989769988 Sequence: RRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD Prediction: +++++++++++-++-----------------------+--- Confidence: 99796584755346001000212000000000000004232 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.