| Detail-Interactions: | |
| RAID ID: | RPI00000030 |
|---|---|
| RNA/Protein Symbol 1: | BCYRN1 |
| RNA/Protein Category 1: | lncRNA |
| RNA/Protein Symbol 2: | EIF4A2(eIF4A) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Electrophoretic Mobility Shift Assay (EMSA), UV Cross-linking Assay |
| Tissue: | HEK293 cells |
| PMID: | 18316401 |
| Detail description: | Inhibits translation. Binds PABP, SRP9/14 heterodimer and eIF4A. |
| The Predicated Binding Sites between BCYRN1 and EIF4A2 | |||||
| By miRanda | Structure | Match Score | Energy Score | BCYRN1 Location | EIF4A2 Location |
| The Predicated Binding Sites between BCYRN1 and EIF4A2 | ||||
| By RIsearch | Structure | BCYRN1 Location | EIF4A2 Location | Score |
| The Predicated Binding Sites of EIF4A2 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|Q14240|IF4A2_HUMAN Eukaryotic initiation factor 4A-II OS=Homo sapiens GN=EIF4A2 PE=1 SV=2 Sequence: MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQ Prediction: ----++++++-----------------------------------+----+---+-+--- Confidence: 523285776832322344678788444667999998884934479658275555444354 Sequence: QRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKV Prediction: -----------------+++++++-+------------------------+--------- Confidence: 428855792947978366879697473884995697848353397657337465369429 Sequence: ILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMF Prediction: ------------------++-++---+--------------+--------+-+------- Confidence: 988898355527364742664842426726476488752357974574225452359289 Sequence: VLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKE Prediction: ----------+--+-----------+-------------------++--+---+------ Confidence: 999998787275554695899649352855989362662787593555585596668234 Sequence: ELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTV Prediction: -------+-------+--------------------------+++----------+---- Confidence: 462667845936295544328758999574949449985622674432737373644839 Sequence: SALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHR Prediction: -----------+-------++++++---------+----------------+++-++--+ Confidence: 277442322265398735476686935734587767867539479964542647355626 Sequence: IGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI Prediction: --++++++++-----------++--+--------------------- Confidence: 53947955954656478266845785597262443953778799899 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|Q14240|IF4A2_HUMAN Eukaryotic initiation factor 4A-II OS=Homo sapiens GN=EIF4A2 PE=1 SV=2 Sequence: MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQ Prediction: -+------------------------------------------------------+--+ Confidence: 435565855676799999999998864477784475485975697499576766434544 Sequence: QRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKV Prediction: ------------------++++++------------------------+-+--------- Confidence: 327979763633865261577587633997895675734243177623415437649549 Sequence: ILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMF Prediction: ----------------+--+------------------+--+------------------ Confidence: 989896236618455232141145743945569575445125853575423865429477 Sequence: VLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKE Prediction: -------------------------+-------+-+------------------------ Confidence: 895473375414656797799448233662966346743697499664747784857334 Sequence: ELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTV Prediction: -------------------------------------------+---------------- Confidence: 443865757878895235329999999858859859997821245867979894766859 Sequence: SALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHR Prediction: -----++-+--+-----------------------------------------------+ Confidence: 846233415453887767524233375775457737777638478936663367562515 Sequence: IGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI Prediction: --++++++++------------------------------------- Confidence: 21958958753267767445732892898696666668777797499 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>Q14240|EIF4A2 Sequence: MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQ Prediction: ---------------------------------------------+--+++++-++++++ Confidence: 010000001100000000000000000000000000001010210642558574787665 Sequence: QRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKV Prediction: -+---+--+-+--+-+++++++++---------------+-------+++++------+- Confidence: 484136215164353867889896344214103200120510412227789810230252 Sequence: ILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMF Prediction: --+---------+-+++++++++----------+-+++++++-++--+++++++++-+-- Confidence: 016122403241848888996752120001110515699899357247887577751711 Sequence: VLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKE Prediction: ---------++----------+-------------+---------++--++--+---+-- Confidence: 102224311673222102202520210010000345114001102664188428331641 Sequence: ELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTV Prediction: -++-+--++------------------------------++++++--+---+-+------ Confidence: 355284257232301200140100140111132242244699998225313506012321 Sequence: SALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHR Prediction: +--+-+--+----------++++++----++---+-----------+-+-+-++----++ Confidence: 644745135203100143278656821236533372121320221153645375444489 Sequence: IGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI Prediction: +++++++++++++++--------------------------+------ Confidence: 689999999999769334302130021014422220140215032000 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MSGGSADYNREHGGPEGMDPDGVIESNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQ Prediction: -++++++-+-----+-+-------------------------------------+++--- Confidence: 244454323010205352101000000000000000000000002000000031444302 Sequence: QRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKV Prediction: ----------++-----+++++++-----------------++--+--+----------- Confidence: 030000000255000028967498200000000000001025921400511000000000 Sequence: ILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMF Prediction: ---------++---+++++++--+--+-+++++-----+-+++-----+------+---- Confidence: 000000222361205744443025113256444331115349302002532000360020 Sequence: VLDEADEMLSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKE Prediction: --++-+--+-------------+----+----++++---------------------+++ Confidence: 004404134100301003120133113510005553002000000001100001000445 Sequence: ELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTV Prediction: -+--+-----------------------------+-+-+-+-+++--------------- Confidence: 353332201011201102110000000000000061505262755010000000000100 Sequence: SALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHR Prediction: -++++++-+--+-------+-++++--++-+-----------------------++-+++ Confidence: 048959625105030001262396412540500302200100000000000110560599 Sequence: IGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI Prediction: ++++++++++-+-----------------+----------------- Confidence: 99999989953402010211001001100401310000000000100 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.