| Detail-Interactions: | |
| RAID ID: | RPI00000029 |
|---|---|
| RNA/Protein Symbol 1: | BCYRN1 |
| RNA/Protein Category 1: | lncRNA |
| RNA/Protein Symbol 2: | EIF4A1(eIF4A) |
| RNA/Protein Category 2: | Protein |
| Validated Method: | Electrophoretic Mobility Shift Assay (EMSA), UV Cross-linking Assay |
| Tissue: | HEK293 cells |
| PMID: | 18316401 |
| Detail description: | Inhibits translation. Binds PABP, SRP9/14 heterodimer and eIF4A. |
| The Predicated Binding Sites between BCYRN1 and EIF4A1 | |||||
| By miRanda | Structure | Match Score | Energy Score | BCYRN1 Location | EIF4A1 Location |
| The Predicated Binding Sites between BCYRN1 and EIF4A1 | ||||
| By RIsearch | Structure | BCYRN1 Location | EIF4A1 Location | Score |
| The Predicated Binding Sites of EIF4A1 | |
| RBPBD | |
| RsiteDB | |
| BindN |
>sp|P60842|IF4A1_HUMAN Eukaryotic initiation factor 4A-I OS=Homo sapiens GN=EIF4A1 PE=1 SV=1 Sequence: MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQ Prediction: ---++++++++++-------------------------------+----+---+-+---- Confidence: 623677897965423555877884446769997998849434896582755554443544 Sequence: RAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVV Prediction: +---------------+++++++-+---------------+--------+---------- Confidence: 488556929479783668796974738849956988592742986573374653594299 Sequence: MALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFV Prediction: -----------------++-+---++--------------+--------+-++---+--- Confidence: 788984555273647426548426476256876987623579745742264652394799 Sequence: LDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEE Prediction: ---------+--+-----------++-----------+------++--+---+------- Confidence: 999987872755546958995353652368693626647874935555855966682344 Sequence: LTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVS Prediction: ------+-------+--------------------------+++----------+----- Confidence: 626678658362955443287589995749494499857326744327373736448382 Sequence: AMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRI Prediction: ----------+-------++++++---------+----------------+++-++--+- Confidence: 764423222653987354766869357345877678675394799645426473556265 Sequence: GRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI Prediction: -++++++++-----------++--+--------------------- Confidence: 3947955954656368244466266276272444963879799799 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| BindN+ |
>sp|P60842|IF4A1_HUMAN Eukaryotic initiation factor 4A-I OS=Homo sapiens GN=EIF4A1 PE=1 SV=1 Sequence: MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQ Prediction: -------------------------------------------------------+--+- Confidence: 524538628589999998999967754877854654869756973995778664446443 Sequence: RAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVV Prediction: -----------------++++++---------------+--------+-+---------- Confidence: 389798637338652615784875339988866766453531766233155486495499 Sequence: MALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFV Prediction: ------------------+------------------+--+------------------- Confidence: 798953457284553122322548639665795854351258626865248764294788 Sequence: LDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEE Prediction: --------------------------------+-+------------------------- Confidence: 854733845246577878995583135527573457545975997657588839562254 Sequence: LTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVS Prediction: ------------------------------------------+----------------- Confidence: 338657478788852354299999997577597498878213448669898947657486 Sequence: AMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRI Prediction: -----+-+--+-----------------------------------------------+- Confidence: 342214144537867565142233656763465265555284778255533675535243 Sequence: GRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI Prediction: -++++++++------------------------------------- Confidence: 2948957743377667445732892798597766668668797599 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| RNAbindR |
>P60842|EIF4A1 Sequence: MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQ Prediction: --------------------------------------------+--+++++-++++++- Confidence: 100000000000000000000000000000000000110103106435685747877654 Sequence: RAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVV Prediction: +---+--+-++-+-+++++++++-----------------------+++++--------- Confidence: 841362151654538678898973442141031000104103122277788102202410 Sequence: MALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFV Prediction: -+---------+-++++++++-------------+++++++-++-++++++++++-+--- Confidence: 151324022318488889967421100012004156998993573578975767526111 Sequence: LDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEE Prediction: --------++----------+-------------+--+------++--++--+---+--- Confidence: 021233116732221022025203200200013451250011025641884383316413 Sequence: LTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVS Prediction: ++-+--++----------+-------------------++++++--+---+-+------+ Confidence: 552842572223012001501011401111322422447988972253135050113116 Sequence: AMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRI Prediction: --+++--+----------++++++----++---+-----------+-+---++----+++ Confidence: 447552462131001432786679212365333721323302311536443754334896 Sequence: GRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI Prediction: ++++++++++++++--------------+-------+---+------ Confidence: 89999999999769334302130021015423221150216042000 *** Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. *** Confidence: from level 0 (lowest) to level 9 (highest). |
| Pprint |
Sequence: MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQ Prediction: ++++++++-+-++--------------------------------------+-+++---- Confidence: 577677452533500000000000000000000000000000010000000316443020 Sequence: RAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVV Prediction: ---------++-----+++++++-----------------++--+--+------------ Confidence: 300000001540000289674981000000010000221248204004120000000000 Sequence: MALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFV Prediction: --------++---++++++---+----+++++++---+++++-----+-----++----- Confidence: 000002223602046445331240022564643431174694130025210004800100 Sequence: LDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEE Prediction: -++----+-------------++---+-----++----------------------++++ Confidence: 034000151002000010100441135000024520031000000031000020004464 Sequence: LTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVS Prediction: +-++-----------------------------+-+-+-+-+------------------ Confidence: 624322210102011021100000000000000404031414230000000000001000 Sequence: AMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRI Prediction: ++++++-+--+-------+-++++--++-+-----------------------++-++++ Confidence: 589495252050310123734964125406002022001000000000002105505998 Sequence: GRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI Prediction: +++++++++++-----------------+--+-------------- Confidence: 9999989943401010000000001200402410201010200110 ***Prediction: binding residues are labeled with '+' and in red; non-binding residues labeled with '-' and in green. ***Confidence: from level 0 (lowest) to level 9 (highest). |
The 'First Node' or 'Second Node' option represents the sub-network of interacting RNA/Protein with the first or second interaction node,
the 'Both the Nodes' option represents the sub-network of interacting RNA/Protein with both of interaction nodes.
The 'First Neighbour' represents the sub-network of direct interacting with the center node,
the 'Second Neighbour' represents the sub-network of direct or second step interacting with the center node.
Interaction sub-network based on the two nodes of this interaction may help the researchers represents all interacting partners immediately.
The Detail page: representing the detail information for RNA-RNA/RNA-Protein interaction;
The Binding page: representing the predicted binding sites and/or constants.
The Network page: representing the interaction sub-network of interacting RNA/Protein.